LOCUS       sequence-124365-        4191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized LPbCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY008 (pcDNA3.1-hPbCpf1)
FEATURES             Location/Qualifiers
     source          1..4191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4057..4104
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4111..4191
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atggagaaca tcttcgacca gtttatcggc aagtacagcc tgtccaagac cctgagattc
       61 gagctgaagc ccgtgggcaa gacagaggac ttcctgaaga tcaacaaggt gtttgagaag
      121 gatcagacca tcgacgatag ctacaatcag gccaagttct attttgattc cctgcaccag
      181 aagtttatcg acgccgccct ggcctccgat aagacatccg agctgtcttt ccagaacttt
      241 gccgacgtgc tggagaagca gaataagatc atcctggata agaagagaga gatgggcgcc
      301 ctgaggaagc gcgacaagaa cgccgtgggc atcgataggc tgcagaagga gatcaatgac
      361 gccgaggata tcatccagaa ggagaaggag aagatctaca aggacgtgcg caccctgttc
      421 gataacgagg ccgagtcttg gaaaacctac tatcaggagc gggaggtgga cggcaagaag
      481 atcaccttca gcaaggccga cctgaagcag aagggcgccg attttctgac agccgccggc
      541 atcctgaagg tgctgaagta tgagttcccc gaggagaagg agaaggagtt tcaggccaag
      601 aaccagccct ccctgttcgt ggaggagaag gagaatcctg gccagaagag gtacatcttc
      661 gactcttttg ataagttcgc cggctatctg accaagtttc agcagacaaa gaagaatctg
      721 tacgcagcag acggcaccag cacagcagtg gccacccgca tcgccgataa ctttatcatc
      781 ttccaccaga ataccaaggt gttccgggac aagtacaaga acaatcacac agacctgggc
      841 ttcgatgagg agaacatctt tgagatcgag aggtataaga attgcctgct gcagcgcgag
      901 atcgagcaca tcaagaatga gaatagctac aacaagatca tcggccggat caataagaag
      961 atcaaggagt atcgggacca gaaggccaag gataccaagc tgacaaagtc cgacttccct
     1021 ttctttaaga acctggataa gcagatcctg ggcgaggtgg agaaggagaa gcagctgatc
     1081 gagaaaaccc gggagaaaac cgaggaggac gtgctgatcg agcggttcaa ggagttcatc
     1141 gagaacaatg aggagaggtt caccgccgcc aagaagctga tgaatgcctt ctgtaacggc
     1201 gagtttgagt ccgagtacga gggcatctat ctgaagaata aggccatcaa cacaatctcc
     1261 cggagatggt tcgtgtctga cagagatttt gagctgaagc tgcctcagca gaagtccaag
     1321 aacaagtctg agaagaatga gccaaaggtg aagaagttca tctccatcgc cgagatcaag
     1381 aacgccgtgg aggagctgga cggcgatatc tttaaggccg tgttctacga caagaagatc
     1441 atcgcccagg gcggctctaa gctggagcag ttcctggtca tctggaagta cgagtttgag
     1501 tatctgttcc gggacatcga gagagagaac ggcgagaagc tgctgggcta tgatagctgc
     1561 ctgaagatcg ccaagcagct gggcatcttc ccacaggaga aggaggcccg cgagaaggca
     1621 accgccgtga tcaagaatta cgccgacgcc ggcctgggca tcttccagat gatgaagtat
     1681 ttttctctgg acgataagga tcggaagaac acccccggcc agctgagcac aaatttctac
     1741 gccgagtatg acggctacta caaggatttc gagtttatca agtactacaa cgagtttagg
     1801 aacttcatca ccaagaagcc tttcgacgag gataagatca agctgaactt tgagaatggc
     1861 gccctgctga agggctggga cgagaacaag gagtacgatt tcatgggcgt gatcctgaag
     1921 aaggagggcc gcctgtatct gggcatcatg cacaagaacc accggaagct gtttcagtcc
     1981 atgggcaatg ccaagggcga caacgccaat agataccaga agatgatcta taagcagatc
     2041 gccgacgcct ctaaggatgt gcccaggctg ctgctgacca gcaagaaggc catggagaag
     2101 ttcaagcctt cccaggagat cctgagaatc aagaaggaga aaaccttcaa gcgggagagc
     2161 aagaactttt ccctgagaga tctgcacgcc ctgatcgagt actataggaa ctgcatccct
     2221 cagtacagca attggtcctt ttatgacttc cagtttcagg ataccggcaa gtaccagaat
     2281 atcaaggagt tcacagacga tgtgcagaag tacggctata agatctcctt tcgcgacatc
     2341 gacgatgagt atatcaatca ggccctgaac gagggcaaga tgtacctgtt cgaggtggtg
     2401 aacaaggata tctataacac caagaatggc tccaagaatc tgcacacact gtactttgag
     2461 cacatcctgt ctgccgagaa cctgaatgac ccagtgttca agctgtctgg catggccgag
     2521 atctttcagc ggcagcccag cgtgaacgaa agagagaaga tcaccacaca gaagaatcag
     2581 tgtatcctgg acaagggcga tagagcctac aagtataggc gctacaccga gaagaagatc
     2641 atgttccaca tgagcctggt gctgaacaca ggcaagggcg agatcaagca ggtgcagttt
     2701 aataagatca tcaaccagag gatcagctcc tctgacaacg agatgagggt gaatgtgatc
     2761 ggcatcgatc gcggcgagaa gaacctgctg tactatagcg tggtgaagca gaatggcgag
     2821 atcatcgagc aggcctccct gaacgagatc aatggcgtga actaccggga caagctgatc
     2881 gagagggaga aggagcgcct gaagaaccgg cagagctgga agcctgtggt gaagatcaag
     2941 gatctgaaga agggctacat ctcccacgtg atccacaaga tctgccagct gatcgagaag
     3001 tattctgcca tcgtggtgct ggaggacctg aatatgagat tcaagcagat caggggagga
     3061 atcgagcgga gcgtgtacca gcagttcgag aaggccctga tcgataagct gggctatctg
     3121 gtgtttaagg acaacaggga tctgagggca ccaggaggcg tgctgaatgg ctaccagctg
     3181 tctgccccct ttgtgagctt cgagaagatg cgcaagcaga ccggcatcct gttctacaca
     3241 caggccgagt ataccagcaa gacagaccca atcaccggct ttcggaagaa cgtgtatatc
     3301 tctaatagcg cctccctgga taagatcaag gaggccgtga agaagttcga cgccatcggc
     3361 tgggatggca aggagcagtc ttacttcttt aagtacaacc cttacaacct ggccgacgag
     3421 aagtataaga actctaccgt gagcaaggag tgggccatct ttgccagcgc cccaagaatc
     3481 cggagacaga agggcgagga cggctactgg aagtatgata gggtgaaagt gaatgaggag
     3541 ttcgagaagc tgctgaaggt ctggaatttt gtgaacccaa aggccacaga tatcaagcag
     3601 gagatcatca agaaggagaa ggcaggcgac ctgcagggag agaaggagct ggatggccgg
     3661 ctgagaaact tttggcactc tttcatctac ctgtttaacc tggtgctgga gctgcgcaat
     3721 tctttcagcc tgcagatcaa gatcaaggca ggagaagtga tcgcagtgga cgagggcgtg
     3781 gacttcatcg ccagcccagt gaagcccttc tttaccacac ccaaccctta catcccctcc
     3841 aacctgtgct ggctggccgt ggagaatgca gacgcaaacg gagcctataa tatcgccagg
     3901 aagggcgtga tgatcctgaa gaagatccgc gagcacgcca agaaggaccc cgagttcaag
     3961 aagctgccaa acctgtttat cagcaatgca gagtgggacg aggcagcccg ggattggggc
     4021 aagtacgcag gcaccacagc cctgaacctg gaccacaaaa ggccggcggc cacgaaaaag
     4081 gccggccagg caaaaaagaa aaagggatcc tacccatacg atgttccaga ttacgcttat
     4141 ccctacgacg tgcctgatta tgcataccca tatgatgtcc ccgactatgc c
//
