LOCUS       sequence-124366-        3885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized SsCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY009 (pcDNA3.1-hSsCpf1)
FEATURES             Location/Qualifiers
     source          1..3885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3751..3798
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3805..3885
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atgcagaccc tgtttgagaa cttcacaaat cagtacccag tgtccaagac cctgcgcttt
       61 gagctgatcc cccagggcaa gacaaaggac ttcatcgagc agaagggcct gctgaagaag
      121 gatgaggacc gggccgagaa gtataagaag gtgaagaaca tcatcgatga gtaccacaag
      181 gacttcatcg agaagtctct gaatggcctg aagctggacg gcctggagaa gtacaagacc
      241 ctgtatctga agcaggagaa ggacgataag gataagaagg cctttgacaa ggagaaggag
      301 aacctgcgca agcagatcgc caatgccttc cggaacaatg agaagtttaa gacactgttc
      361 gccaaggagc tgatcaagaa cgatctgatg tctttcgcct gcgaggagga caagaagaat
      421 gtgaaggagt ttgaggcctt caccacatac ttcaccggct tccaccagaa ccgcgccaat
      481 atgtacgtgg ccgatgagaa gagaacagcc atcgccagca ggctgatcca cgagaacctg
      541 ccaaagttta tcgacaatat caagatcttc gagaagatga agaaggaggc ccccgagctg
      601 ctgtctcctt tcaaccagac cctgaaggat atgaaggacg tgatcaaggg caccacactg
      661 gaggagatct ttagcctgga ttatttcaac aagaccctga cacagagcgg catcgacatc
      721 tacaattccg tgatcggcgg cagaacccct gaggagggca agacaaagat caagggcctg
      781 aacgagtaca tcaataccga cttcaaccag aagcagacag acaagaagaa gcggcagcca
      841 aagttcaagc agctgtataa gcagatcctg agcgataggc agagcctgtc ctttatcgcc
      901 gaggccttca agaacgacac cgagatcctg gaggccatcg agaagtttta cgtgaatgag
      961 ctgctgcact tcagcaatga gggcaagtcc acaaacgtgc tggacgccat caagaatgcc
     1021 gtgtctaacc tggagagctt taacctgacc aagatgtatt tccgctccgg cgcctctctg
     1081 acagacgtga gccggaaggt gtttggcgag tggagcatca tcaatagagc cctggacaac
     1141 tactatgcca ccacatatcc aatcaagccc agagagaagt ctgagaagta cgaggagagg
     1201 aaggagaagt ggctgaagca ggacttcaac gtgagcctga tccagaccgc catcgatgag
     1261 tacgacaacg agacagtgaa gggcaagaac agcggcaaag tgatcgccga ttattttgcc
     1321 aagttctgcg acgataagga gacagacctg atccagaagg tgaacgaggg ctacatcgcc
     1381 gtgaaggatc tgctgaatac accctgtcct gagaacgaga agctgggcag caataaggac
     1441 caggtgaagc agatcaaggc ctttatggat tctatcatgg acatcatgca cttcgtgcgc
     1501 cccctgagcc tgaaggatac cgacaaggag aaggatgaga cattctactc cctgttcaca
     1561 cctctgtacg accacctgac ccagacaatc gccctgtata acaaggtgcg gaactatctg
     1621 acccagaagc cttacagcac agagaagatc aagctgaact tcgagaacag caccctgctg
     1681 ggcggctggg atctgaataa ggagacagac aacacagcca tcatcctgag gaaggataac
     1741 ctgtactatc tgggcatcat ggacaagagg cacaatcgca tctttcggaa cgtgcccaag
     1801 gccgataaga aggacttctg ctacgagaag atggtgtata agctgctgcc tggcgccaac
     1861 aagatgctgc caaaggtgtt cttttctcag agcagaatcc aggagtttac cccttccgcc
     1921 aagctgctgg agaactacgc caatgagaca cacaagaagg gcgataattt caacctgaat
     1981 cactgtcaca agctgatcga tttctttaag gactctatca acaagcacga ggattggaag
     2041 aatttcgact ttaggttcag cgccacctcc acctacgccg acctgagcgg cttttaccac
     2101 gaggtggagc accagggcta caagatctct tttcagagcg tggccgattc cttcatcgac
     2161 gatctggtga acgagggcaa gctgtacctg ttccagatct ataataagga cttttcccca
     2221 ttctctaagg gcaagcccaa cctgcacacc ctgtactgga agatgctgtt tgatgagaac
     2281 aatctgaagg acgtggtgta taagctgaat ggcgaggccg aggtgttcta ccgcaagaag
     2341 agcattgccg agaagaacac cacaatccac aaggccaatg agtccatcat caacaagaat
     2401 cctgataacc caaaggccac cagcaccttc aactatgata tcgtgaagga caagagatac
     2461 accatcgaca agtttcagtt ccacatccca atcacaatga actttaaggc cgagggcatc
     2521 ttcaacatga atcagagggt gaatcagttc ctgaaggcca atcccgatat caacatcatc
     2581 ggcatcgaca gaggcgagag gcacctgctg tactatgccc tgatcaacca gaagggcaag
     2641 atcctgaagc aggataccct gaatgtgatc gccaacgaga agcagaaggt ggactaccac
     2701 aatctgctgg ataagaagga gggcgaccgc gcaaccgcaa ggcaggagtg gggcgtgatc
     2761 gagacaatca aggagctgaa ggagggctat ctgtcccagg tcatccacaa gctgaccgat
     2821 ctgatgatcg agaacaatgc catcatcgtg atggaggacc tgaactttgg cttcaagcgg
     2881 ggcagacaga aggtggagaa gcaggtgtat cagaagtttg agaagatgct gatcgataag
     2941 ctgaattacc tggtggacaa gaataagaag gcaaacgagc tgggaggcct gctgaacgca
     3001 ttccagctgg ccaataagtt tgagtccttc cagaagatgg gcaagcagaa cggctttatc
     3061 ttctacgtgc ccgcctggaa tacctctaag acagatcctg ccaccggctt tatcgacttc
     3121 ctgaagcccc gctatgagaa cctgaatcag gccaaggatt tctttgagaa gtttgactct
     3181 atccggctga acagcaaggc cgattacttt gagttcgcct ttgacttcaa gaatttcacc
     3241 gagaaggccg atggcggcag aaccaagtgg acagtgtgca ccacaaacga ggacagatat
     3301 gcctggaata gggccctgaa caataacagg ggcagccagg agaagtacga catcacagcc
     3361 gagctgaagt ccctgttcga tggcaaggtg gactataagt ctggcaagga tctgaagcag
     3421 cagatcgcca gccaggagtc cgccgacttc tttaaggccc tgatgaagaa cctgtccatc
     3481 accctgtctc tgagacacaa taacggcgag aagggcgata atgagcagga ctacatcctg
     3541 tcccctgtgg ccgattctaa gggccgcttc tttgactccc ggaaggccga cgatgacatg
     3601 ccaaagaatg ccgacgccaa cggcgcctat cacatcgccc tgaagggcct gtggtgtctg
     3661 gagcagatca gcaagaccga tgacctgaag aaggtgaagc tggccatctc caacaaggag
     3721 tggctggagt tcgtgcagac actgaagggc aaaaggccgg cggccacgaa aaaggccggc
     3781 caggcaaaaa agaaaaaggg atcctaccca tacgatgttc cagattacgc ttatccctac
     3841 gacgtgcctg attatgcata cccatatgat gtccccgact atgcc
//
