LOCUS       sequence-124369-        3852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized CMtCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY012 (pcDNA3.1-hCMtCpf1)
FEATURES             Location/Qualifiers
     source          1..3852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3715..3762
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3769..3849
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atgaacaatt acgacgagtt caccaagctg tatcctatcc agaaaaccat ccggtttgag
       61 ctgaagccac agggcagaac catggagcac ctggagacat tcaacttctt tgaggaggac
      121 cgggatagag ccgagaagta taagatcctg aaggaggcca tcgacgagta ccacaagaag
      181 tttatcgatg agcacctgac caatatgtcc ctggattgga actctctgaa gcagatcagc
      241 gagaagtact ataagagcag ggaggagaag gacaagaagg tgttcctgtc cgagcagaag
      301 aggatgcgcc aggagatcgt gtctgagttt aagaaggacg atcgcttcaa ggacctgttt
      361 tccaagaagc tgttctctga gctgctgaag gaggagatct acaagaaggg caaccaccag
      421 gagatcgacg ccctgaagag cttcgataag ttttccggct atttcatcgg cctgcacgag
      481 aataggaaga acatgtactc cgacggcgat gagatcaccg ccatctccaa tcgcatcgtg
      541 aatgagaact tccccaagtt tctggataac ctgcagaagt accaggaggc caggaagaag
      601 tatcctgagt ggatcatcaa ggccgagagc gccctggtgg cccacaatat caagatggac
      661 gaggtgttct ccctggagta ctttaataag gtgctgaacc aggagggcat ccagcggtac
      721 aacctggccc tgggcggcta tgtgaccaag agcggcgaga agatgatggg cctgaatgat
      781 gccctgaacc tggcccacca gtccgagaag agctccaagg gcagaatcca catgaccccc
      841 ctgttcaagc agatcctgtc cgagaaggag tccttctctt acatccccga cgtgtttaca
      901 gaggattctc agctgctgcc tagcatcggc ggcttctttg cccagatcga gaatgacaag
      961 gatggcaaca tcttcgaccg ggccctggag ctgatctcta gctacgccga gtatgatacc
     1021 gagcggatct atatcagaca ggccgacatc aatagagtgt ccaacgtgat ctttggagag
     1081 tggggcaccc tgggaggcct gatgagggag tacaaggccg actctatcaa tgatatcaac
     1141 ctggagcgca catgcaagaa ggtggacaag tggctggatt ctaaggagtt tgccctgagc
     1201 gatgtgctgg aggccatcaa gaggaccggc aacaatgacg ccttcaacga gtatatctcc
     1261 aagatgcgga cagccagaga gaagatcgat gccgcccgca aggagatgaa gttcatcagc
     1321 gagaagatct ccggcgatga ggagtctatc cacatcatca agaccctgct ggacagcgtg
     1381 cagcagttcc tgcacttctt taatctgttt aaggcaaggc aggacatccc actggatgga
     1441 gccttctacg ccgagtttga cgaggtgcac agcaagctgt ttgccatcgt gcccctgtat
     1501 aacaaggtgc ggaactatct gaccaagaac aatctgaaca caaagaagat caagctgaat
     1561 ttcaagaacc ctacactggc caatggctgg gaccagaaca aggtgtacga ttatgcctcc
     1621 ctgatctttc tgcgggacgg caattactat ctgggcatca tcaatcctaa gagaaagaag
     1681 aacatcaagt tcgagcaggg ctctggcaac ggccccttct accggaagat ggtgtataag
     1741 cagatccccg gccctaataa gaacctgcca agagtgttcc tgacctccac aaagggcaag
     1801 aaggagtata agccctctaa ggagatcatc gagggctacg aggccgacaa gcacatcagg
     1861 ggcgataagt tcgacctgga tttttgtcac aagctgatcg atttctttaa ggagtccatc
     1921 gagaagcaca aggactggtc taagttcaac ttctacttca gcccaaccga gagctatggc
     1981 gacatctctg agttctacct ggatgtggag aagcagggct atcgcatgca ctttgagaat
     2041 atcagcgccg agacaatcga cgagtatgtg gagaagggcg atctgtttct gttccagatc
     2101 tacaacaagg attttgtgaa ggccgccacc ggcaagaagg acatgcacac aatctactgg
     2161 aatgccgcct tcagccccga gaacctgcag gacgtggtgg tgaagctgaa cggcgaggcc
     2221 gagctgtttt atagggacaa gtccgatatc aaggagatcg tgcaccgcga gggcgagatc
     2281 ctggtgaata ggacctacaa cggccgcaca ccagtgcccg acaagatcca caagaagctg
     2341 accgattatc acaatggccg gacaaaggac ctgggcgagg ccaaggagta cctggataag
     2401 gtgagatact tcaaggccca ctatgacatc accaaggatc ggagatacct gaacgacaag
     2461 atctatttcc acgtgcctct gaccctgaac ttcaaggcca acggcaagaa gaatctgaac
     2521 aagatggtca tcgagaagtt cctgtccgat gagaaggccc acatcatcgg catcgacagg
     2581 ggcgagcgca atctgctgta ctattccatc atcgacaggt ctggcaagat catcgatcag
     2641 cagagcctga atgtgatcga cggctttgat tatcgggaga agctgaacca gagagagatc
     2701 gagatgaagg atgcccgcca gtcttggaac gccatcggca agatcaagga cctgaaggag
     2761 ggctacctga gcaaggccgt gcacgagatc accaagatgg ccatccagta taatgccatc
     2821 gtggtcatgg aggagctgaa ctacggcttc aagcggggcc ggttcaaggt ggagaagcag
     2881 atctatcaga agttcgagaa tatgctgatc gataagatga actacctggt gtttaaggac
     2941 gcacctgatg agtccccagg aggcgtgctg aatgcctacc agctgacaaa cccactggag
     3001 tctttcgcca agctgggcaa gcagaccggc atcctgtttt acgtgccagc cgcctataca
     3061 tccaagatcg accccaccac aggcttcgtg aatctgttta acacctcctc taagacaaac
     3121 gcccaggagc ggaaggagtt cctgcagaag tttgagagca tctcctattc tgccaaggat
     3181 ggcggcatct ttgccttcgc ctttgactac agaaagttcg gcaccagcaa gacagatcac
     3241 aagaacgtgt ggaccgccta tacaaacggc gagaggatgc gctacatcaa ggagaagaag
     3301 cggaatgagc tgtttgaccc ttctaaggag atcaaggagg ccctgaccag ctccggcatc
     3361 aagtacgatg gcggccagaa catcctgcca gacatcctga ggagcaacaa taacggcctg
     3421 atctacacaa tgtattctag cttcatcgcc gccatccaga tgcgcgtgta cgacggcaag
     3481 gaggattata tcatcagccc catcaagaac tccaagggcg agttctttag gaccgacccc
     3541 aagaggcgcg agctgcctat cgacgccgat gccaatggcg cctacaacat cgccctgagg
     3601 ggagagctga caatgagggc aatcgcagag aagttcgacc ctgatagcga gaagatggcc
     3661 aagctggagc tgaagcacaa ggattggttc gagtttatgc agaccagagg cgacaaaagg
     3721 ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggatccta cccatacgat
     3781 gttccagatt acgcttatcc ctacgacgtg cctgattatg catacccata tgatgtcccc
     3841 gactatgcct aa
//
