LOCUS       sequence-124370-        3984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized EeCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY013 (pcDNA3.1-hEeCpf1)
FEATURES             Location/Qualifiers
     source          1..3984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3847..3894
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3901..3981
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atgaacggca ataggtccat cgtgtaccgc gagttcgtgg gcgtgatccc cgtggccaag
       61 accctgagga atgagctgcg ccctgtgggc cacacacagg agcacatcat ccagaacggc
      121 ctgatccagg aggacgagct gcggcaggag aagagcaccg agctgaagaa catcatggac
      181 gattactata gagagtacat cgataagtct ctgagcggcg tgaccgacct ggacttcacc
      241 ctgctgttcg agctgatgaa cctggtgcag agctccccct ccaaggacaa taagaaggcc
      301 ctggagaagg agcagtctaa gatgagggag cagatctgca cccacctgca gtccgactct
      361 aactacaaga atatctttaa cgccaagctg ctgaaggaga tcctgcctga tttcatcaag
      421 aactacaatc agtatgacgt gaaggataag gccggcaagc tggagacact ggccctgttt
      481 aatggcttca gcacatactt taccgacttc tttgagaaga ggaagaacgt gttcaccaag
      541 gaggccgtga gcacatccat cgcctaccgc atcgtgcacg agaactccct gatcttcctg
      601 gccaatatga cctcttataa gaagatcagc gagaaggccc tggatgagat cgaagtgatc
      661 gagaagaaca atcaggacaa gatgggcgat tgggagctga atcagatctt taaccctgac
      721 ttctacaata tggtgctgat ccagtccggc atcgacttct acaacgagat ctgcggcgtg
      781 gtgaatgccc acatgaacct gtactgtcag cagaccaaga acaattataa cctgttcaag
      841 atgcggaagc tgcacaagca gatcctggcc tacaccagca ccagcttcga ggtgcccaag
      901 atgttcgagg acgatatgag cgtgtataac gccgtgaacg ccttcatcga cgagacagag
      961 aagggcaaca tcatcggcaa gctgaaggat atcgtgaata agtacgacga gctggatgag
     1021 aagagaatct atatcagcaa ggacttttac gagacactga gctgcttcat gtccggcaac
     1081 tggaatctga tcacaggctg cgtggagaac ttctacgatg agaacatcca cgccaagggc
     1141 aagtccaagg aggagaaggt gaagaaggcc gtgaaggagg acaagtacaa gtctatcaat
     1201 gacgtgaacg atctggtgga gaagtatatc gatgagaagg agaggaatga gttcaagaac
     1261 agcaatgcca agcagtacat ccgcgagatc tccaacatca tcaccgacac agagacagcc
     1321 cacctggagt atgacgatca catctctctg atcgagagcg aggagaaggc cgacgagatg
     1381 aagaagcggc tggatatgta tatgaacatg taccactggg ccaaggcctt tatcgtggac
     1441 gaggtgctgg acagagatga gatgttctac agcgatatcg acgatatcta taatatcctg
     1501 gagaacatcg tgccactgta taatcgggtg agaaactacg tgacccagaa gccctacaac
     1561 tctaagaaga tcaagctgaa tttccagagc cctacactgg ccaatggctg gtcccagtct
     1621 aaggagttcg acaacaatgc catcatcctg atcagagata acaagtacta tctggccatc
     1681 ttcaatgcca agaacaagcc agacaagaag atcatccagg gcaactccga taagaagaac
     1741 gacaacgatt acaagaagat ggtgtataac ctgctgccag gcgccaacaa gatgctgccc
     1801 aaggtgtttc tgtctaagaa gggcatcgag acattcaagc cctccgacta tatcatctct
     1861 ggctacaacg cccacaagca catcaagaca agcgagaatt ttgatatctc cttctgtcgg
     1921 gacctgatcg attacttcaa gaacagcatc gagaagcacg ccgagtggag aaagtatgag
     1981 ttcaagtttt ccgccaccga cagctactcc gatatctctg agttctatcg ggaggtggag
     2041 atgcagggct acagaatcga ctggacatat atcagcgagg ccgacatcaa caagctggat
     2101 gaggagggca agatctatct gtttcagatc tacaataagg atttcgccga gaacagcacc
     2161 ggcaaggaga atctgcacac aatgtacttt aagaacatct tctccgagga gaatctgaag
     2221 gacatcatca tcaagctgaa cggccaggcc gagctgtttt atcggagagc ctctgtgaag
     2281 aatcccgtga agcacaagaa ggatagcgtg ctggtgaaca agacctacaa gaatcagctg
     2341 gacaacggcg acgtggtgag aatccccatc cctgacgata tctataacga gatctacaag
     2401 atgtataatg gctacatcaa ggagtccgac ctgtctgagg ccgccaagga gtacctggat
     2461 aaggtggagg tgaggaccgc ccagaaggac atcgtgaagg attaccgcta tacagtggac
     2521 aagtacttca tccacacacc tatcaccatc aactataagg tgaccgcccg caacaatgtg
     2581 aatgatatgg tggtgaagta catcgcccag aacgacgata tccacgtgat cggcatcgac
     2641 cggggcgaga gaaacctgat ctacatctcc gtgatcgatt ctcacggcaa catcgtgaag
     2701 cagaaatcct acaacatcct gaacaactac gactacaaga agaagctggt ggagaaggag
     2761 aaaacccggg agtacgccag aaagaactgg aagagcatcg gcaatatcaa ggagctgaag
     2821 gagggctata tctccggcgt ggtgcacgag atcgccatgc tgatcgtgga gtacaacgcc
     2881 atcatcgcca tggaggacct gaattatggc tttaagaggg gccgcttcaa ggtggagcgg
     2941 caggtgtacc agaagtttga gagcatgctg atcaataagc tgaactattt cgccagcaag
     3001 gagaagtccg tggacgagcc aggaggcctg ctgaagggct atcagctgac ctacgtgccc
     3061 gataatatca agaacctggg caagcagtgc ggcgtgatct tttacgtgcc tgccgccttc
     3121 accagcaaga tcgacccatc cacaggcttt atctctgcct tcaactttaa gtctatcagc
     3181 acaaatgcct ctcggaagca gttctttatg cagtttgacg agatcagata ctgtgccgag
     3241 aaggatatgt tcagctttgg cttcgactac aacaacttcg atacctacaa catcacaatg
     3301 ggcaagacac agtggaccgt gtatacaaac ggcgagagac tgcagtctga gttcaacaat
     3361 gccaggcgca ccggcaagac aaagagcatc aatctgacag agacaatcaa gctgctgctg
     3421 gaggacaatg agatcaacta cgccgacggc cacgatatca ggatcgatat ggagaagatg
     3481 gacgaggata agaagagcga gttctttgcc cagctgctga gcctgtataa gctgaccgtg
     3541 cagatgcgca attcctatac agaggccgag gagcaggaga acggcatctc ttacgacaag
     3601 atcatcagcc ctgtgatcaa tgatgagggc gagttctttg actccgataa ctataaggag
     3661 tctgacgata aggagtgcaa gatgccaaag gacgccgatg ccaacggcgc ctactgtatc
     3721 gccctgaagg gcctgtatga ggtgctgaag atcaagagcg agtggaccga ggacggcttt
     3781 gataggaatt gcctgaagct gccacacgca gagtggctgg acttcatcca gaacaagcgg
     3841 tacgagaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc
     3901 tacccatacg atgttccaga ttacgcttat ccctacgacg tgcctgatta tgcataccca
     3961 tatgatgtcc ccgactatgc ctaa
//
