LOCUS       sequence-124372-        3927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized LiCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY015 (pcDNA3.1-hLiCpf1)
FEATURES             Location/Qualifiers
     source          1..3927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3790..3837
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3844..3924
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atggaggact attccggctt tgtgaacatc tactctatcc agaaaaccct gaggttcgag
       61 ctgaagccag tgggcaagac actggagcac atcgagaaga agggcttcct gaagaaggac
      121 aagatccggg ccgaggatta caaggccgtg aagaagatca tcgataagta ccacagagcc
      181 tatatcgagg aggtgtttga ttccgtgctg caccagaaga agaagaagga caagacccgc
      241 ttttctacac agttcatcaa ggagatcaag gagttcagcg agctgtacta taagaccgag
      301 aagaacatcc ccgacaagga gaggctggag gccctgagcg agaagctgcg caagatgctg
      361 gtgggcgcct ttaagggcga gttctccgag gaggtggccg agaagtataa gaacctgttt
      421 tctaaggagc tgatcaggaa tgagatcgag aagttctgcg agacagacga ggagcgcaag
      481 caggtgtcta acttcaagag cttcaccaca tactttaccg gcttccactc caacaggcag
      541 aatatctatt ccgacgagaa gaagtctaca gccatcggct accgcatcat ccaccagaac
      601 ctgcctaagt tcctggataa tctgaagatc atcgagtcca tccagcggcg gttcaaggac
      661 ttcccatggt ctgatctgaa gaagaacctg aagaagatcg ataagaatat caagctgacc
      721 gagtacttca gcatcgacgg cttcgtgaac gtgctgaatc agaagggcat cgatgcctac
      781 aacacaatcc tgggcggcaa gtccgaggag tctggcgaga agatccaggg cctgaacgag
      841 tacatcaatc tgtatcggca gaagaacaat atcgacagaa agaacctgcc caatgtgaag
      901 atcctgttta agcagatcct gggcgatagg gagacaaaga gctttatccc tgaggccttc
      961 ccagacgatc agtccgtgct gaactctatc acagagttcg ccaagtacct gaagctggat
     1021 aagaagaaga agagcatcat cgccgagctg aagaagtttc tgagctcctt caatcgctac
     1081 gagctggacg gcatctatct ggccaacgat aatagcctgg cctctatcag caccttcctg
     1141 tttgacgatt ggtcctttat caagaagtcc gtgtctttca agtatgacga gtccgtgggc
     1201 gaccccaaga agaagatcaa gtctcccctg aagtacgaga aggagaagga gaagtggctg
     1261 aagcagaagt actatacaat ctctttcctg aacgatgcca tcgagagcta ttccaagtct
     1321 caggacgaga agagggtgaa gatccgcctg gaggcctact ttgccgagtt caagagcaag
     1381 gacgatgcca agaagcagtt cgacctgctg gagaggatcg aggaggccta tgccatcgtg
     1441 gagcctctgc tgggagcaga gtacccaagg gaccgcaacc tgaaggccga taagaaggaa
     1501 gtgggcaaga tcaaggactt cctggatagc atcaagtccc tgcagttctt tctgaagcct
     1561 ctgctgtccg ccgagatctt tgacgagaag gatctgggct tctacaatca gctggagggc
     1621 tactatgagg agatcgattc tatcggccac ctgtataaca aggtgcggaa ttatctgacc
     1681 ggcaagatct acagcaagga gaagtttaag ctgaacttcg agaacagcac cctgctgaag
     1741 ggctgggacg agaaccggga ggtggccaat ctgtgcgtga tcttcagaga ggaccagaag
     1801 tactatctgg gcgtgatgga taaggagaac aataccatcc tgtccgacat ccccaaggtg
     1861 aagcctaacg agctgtttta cgagaagatg gtgtataagc tgatccccac acctcacatg
     1921 cagctgcccc ggatcatctt ctctagcgac aacctgtcta tctataatcc tagcaagtcc
     1981 atcctgaaga tcagagaggc caagagcttt aaggagggca agaacttcaa gctgaaggac
     2041 tgtcacaagt ttatcgattt ctacaaggag tctatcagca agaatgagga ctggagcaga
     2101 ttcgacttca agttcagcaa gaccagcagc tacgagaaca tcagcgagtt ttaccgggag
     2161 gtggagagac agggctataa cctggacttc aagaaggtgt ctaagttcta catcgacagc
     2221 ctggtggagg atggcaagct gtacctgttc cagatctata acaaggactt ttctatcttc
     2281 agcaagggca agcccaatct gcacaccatc tattttcggt ccctgttctc taaggagaac
     2341 ctgaaggacg tgtgcctgaa gctgaatggc gaggccgaga tgttctttcg gaagaagtcc
     2401 atcaactacg atgagaagaa gaagcgggag ggccaccacc ccgagctgtt tgagaagctg
     2461 aagtatccta tcctgaagga caagagatac agcgaggata agtttcagtt ccacctgccc
     2521 atcagcctga acttcaagtc caaggagcgg ctgaacttta atctgaaagt gaatgagttc
     2581 ctgaagagaa acaaggacat caatatcatc ggcatcgatc ggggcgagag aaacctgctg
     2641 tacctggtca tgatcaatca gaagggcgag atcctgaagc agaccctgct ggacagcatg
     2701 cagtccggca agggccggcc tgagatcaac tacaaggaga agctgcagga gaaggagatc
     2761 gagagggata aggcccgcaa gagctggggc acagtggaga atatcaagga gctgaaggag
     2821 ggctatctgt ctatcgtgat ccaccagatc agcaagctga tggtggagaa caatgccatc
     2881 gtggtgctgg aggacctgaa catcggcttt aagcggggca gacagaaggt ggagcggcag
     2941 gtgtaccaga agttcgagaa gatgctgatc gataagctga actttctggt gttcaaggag
     3001 aataagccaa ccgagccagg aggcgtgctg aaggcctatc agctgacaga cgagtttcag
     3061 tctttcgaga agctgagcaa gcagaccggc tttctgttct acgtgccaag ctggaacacc
     3121 tccaagatcg accccagaac aggctttatc gatttcctgc accctgccta cgagaatatc
     3181 gagaaggcca agcagtggat caacaagttt gattccatca ggttcaattc taagatggac
     3241 tggtttgagt tcaccgccga tacacgcaag ttttccgaga acctgatgct gggcaagaat
     3301 cgggtgtggg tcatctgcac cacaaatgtg gagcggtact tcaccagcaa gaccgccaac
     3361 agctccatcc agtacaatag catccagatc accgagaagc tgaaggagct gtttgtggac
     3421 atccctttca gcaacggcca ggatctgaag ccagagatcc tgaggaagaa tgacgccgtg
     3481 ttctttaaga gcctgctgtt ttacatcaag accacactgt ccctgcgcca gaacaatggc
     3541 aagaagggcg aggaggagaa ggacttcatc ctgagcccag tggtggattc caagggccgg
     3601 ttctttaact ctctggaggc cagcgacgat gagcccaagg acgccgatgc caatggcgcc
     3661 taccacatcg ccctgaaggg cctgatgaac ctgctggtgc tgaatgagac aaaggaggag
     3721 aacctgagca gaccaaagtg gaagatcaag aataaggact ggctggagtt cgtgtgggag
     3781 aggaaccgca aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga
     3841 tcctacccat acgatgttcc agattacgct tatccctacg acgtgcctga ttatgcatac
     3901 ccatatgatg tccccgacta tgcctaa
//
