LOCUS       sequence-124374-        3918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized PcCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY017 (pcDNA3.1-hPcCpf1)
FEATURES             Location/Qualifiers
     source          1..3918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3781..3828
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3835..3915
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atggacagcc tgaaggattt caccaacctg taccccgtgt ccaagacact gcggtttgag
       61 ctgaagcctg tgggcaagac cctggagaat atcgagaagg ccggcatcct gaaggaggat
      121 gagcacagag ccgagagcta ccggagagtg aagaagatca tcgatacata tcacaaggtg
      181 ttcatcgaca gctccctgga gaacatggcc aagatgggca tcgagaatga gatcaaggcc
      241 atgctgcagt ccttttgcga gctgtataag aaggaccaca ggaccgaggg agaggacaag
      301 gccctggata agatcagggc cgtgctgagg ggcctgatcg tgggagcctt caccggcgtg
      361 tgcggccggc gggagaacac agtgcagaat gagaagtatg agagcctgtt taaggagaag
      421 ctgatcaagg agatcctgcc agatttcgtg ctgtctacag aggccgagtc cctgcccttt
      481 tctgtggagg aggccaccag aagcctgaag gagttcgact cctttacatc ttacttcgcc
      541 ggcttttatg agaaccggaa gaatatctac tctaccaagc cccagagcac agccatcgcc
      601 tatagactga tccacgagaa cctgcctaag ttcatcgata atatcctggt gtttcagaag
      661 atcaaggagc caatcgccaa ggagctggag cacatcaggg cagacttcag cgccggcggc
      721 tacatcaaga aggatgagcg cctggaggac atcttttccc tgaactacta tatccacgtg
      781 ctgtctcagg ccggcatcga gaagtacaat gccctgatcg gcaagatcgt gaccgagggc
      841 gatggcgaga tgaagggcct gaacgagcac atcaacctgt ataatcagca gaggggccgc
      901 gaggaccggc tgccactgtt cagacccctg tataagcaga tcctgtctga tagggagcag
      961 ctgtcctatc tgccagagtc tttcgagaag gacgaggagc tgctgagggc cctgaaggag
     1021 ttttacgatc acatcgcaga ggacatcctg ggaaggaccc agcagctgat gacaagcatc
     1081 tccgagtacg atctgtcccg gatctatgtg agaaacgata gccagctgac cgacatctcc
     1141 aagaagatgc tgggcgattg gaatgccatc tacatggccc gggagagagc ctatgaccac
     1201 gagcaggccc ccaagcgcat cacagccaag tacgagaggg accgcatcaa ggccctgaag
     1261 ggcgaggagt ctatcagcct ggccaacctg aacagctgca tcgccttcct ggacaacgtg
     1321 agggattgtc gcgtggacac ctatctgtct acactgggac agaaggaggg acctcacggc
     1381 ctgagcaacc tggtggagaa cgtgttcgcc tcctaccacg aggccgagca gctgctgtct
     1441 tttccctatc ctgaggagaa caatctgatc caggacaagg ataacgtggt gctgatcaag
     1501 aacctgctgg ataatatcag cgacctgcag aggttcctga agccactgtg gggcatgggc
     1561 gatgagcccg acaaggatga gaggttttac ggcgagtaca attatatcag gggcgccctg
     1621 gaccaggtca tccctctgta taacaaggtg cggaattatc tgacccgcaa gccatactcc
     1681 acacgcaagg tgaagctgaa cttcggcaat agccagctgc tgtccggctg ggataggaac
     1741 aaggagaagg acaattcttg cgtgatcctg cgcaagggcc agaacttcta cctggccatc
     1801 atgaacaatc ggcacaagcg gagcttcgag aataagatgc tgcccgagta taaggagggc
     1861 gagccttact tcgagaagat ggattataag tttctgccag accccaacaa gatgctgccc
     1921 aaggtgttcc tgtctaagaa gggcatcgag atctacaagc ctagcccaaa gctgctggag
     1981 cagtatggcc acggcaccca caagaagggc gataccttca gcatggacga tctgcacgag
     2041 ctgatcgact tctttaagca ctccatcgag gcccacgagg attggaagca gttcggcttt
     2101 aagttcagcg acaccgccac atacgagaac gtgagcagct tctaccggga ggtggaggac
     2161 cagggctaca agctgtcttt tagaaaggtg tccgagtctt acgtgtatag cctgatcgat
     2221 cagggcaagc tgtacctgtt ccagatctat aacaaggact ttagcccttg ttccaagggc
     2281 accccaaatc tgcacacact gtactggcgg atgctgttcg atgagagaaa cctggccgac
     2341 gtgatctata agctggatgg caaggccgag atcttctttc gggagaagtc cctgaagaat
     2401 gaccacccaa cccaccctgc aggcaagccc atcaagaaga agagccggca gaagaagggc
     2461 gaggagagcc tgttcgagta cgatctggtg aaggaccgga gatataccat ggataagttt
     2521 cagttccacg tgccaatcac aatgaacttt aagtgctctg ccggcagcaa ggtgaacgac
     2581 atggtgaatg cccacatcag ggaggccaag gacatgcacg tgatcggcat cgataggggc
     2641 gagcgcaatc tgctgtatat ctgcgtgatc gacagccgcg gcaccatcct ggatcagatc
     2701 tccctgaaca caatcaatga catcgattat cacgatctgc tggagtccag ggacaaggat
     2761 cgccagcagg agcacaggaa ctggcagacc atcgagggca tcaaggagct gaagcagggc
     2821 tacctgtctc aggccgtgca ccgcatcgcc gagctgatgg tggcctataa ggccgtggtg
     2881 gccctggagg acctgaacat gggcttcaag cggggcagac agaaggtgga gagcagcgtg
     2941 taccagcagt ttgagaagca gctgatcgac aagctgaatt atctggtgga taagaagaag
     3001 cggcccgagg acatcggagg cctgctgaga gcctaccagt tcaccgcccc tttcaagagc
     3061 tttaaggaga tgggcaagca gaacggcttt ctgttctata tccctgcctg gaacacatcc
     3121 aatatcgacc caaccacagg cttcgtgaac ctgtttcacg tgcagtacga gaatgtggat
     3181 aaggccaaga gcttctttca gaagttcgac agcatctcct acaaccctaa gaaggattgg
     3241 tttgagttcg cctttgacta taagaacttc accaagaagg ccgagggctc taggagcatg
     3301 tggattctgt gcacccacgg ctcccggatc aagaacttca gaaattctca gaagaatggc
     3361 cagtgggata gcgaggagtt tgccctgacc gaggccttca agtccctgtt tgtgcggtac
     3421 gagatcgatt ataccgccga cctgaaaacc gccatcgtgg acgagaagca gaaggatttc
     3481 tttgtggacc tgctgaagct gttcaagctg accgtgcaga tgagaaactc ctggaaggag
     3541 aaggacctgg attacctgat ctctccagtg gccggcgccg atggcaggtt ctttgacaca
     3601 cgcgagggca ataagagcct gcccaaggac gcagatgcaa acggagccta taatatcgcc
     3661 ctgaagggcc tgtgggcact gaggcagatc agacagacct ccgagggcgg caagctgaag
     3721 ctggccatct ctaacaagga gtggctgcag tttgtgcagg agagatccta cgagaaggac
     3781 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaaggg atcctaccca
     3841 tacgatgttc cagattacgc ttatccctac gacgtgcctg attatgcata cccatatgat
     3901 gtccccgact atgcctaa
//
