LOCUS       sequence-124375-        4107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized PdCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY018 (pcDNA3.1-hPdCpf1)
FEATURES             Location/Qualifiers
     source          1..4107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3970..4017
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4024..4104
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atggagaact atcaggagtt caccaacctg tttcagctga ataagacact gagattcgag
       61 ctgaagccca tcggcaagac ctgcgagctg ctggaggagg gcaagatctt cgccagcggc
      121 tcctttctgg agaaggacaa ggtgagggcc gataacgtga gctacgtgaa gaaggagatc
      181 gacaagaagc acaagatctt tatcgaggag acactgagct ccttctctat cagcaacgat
      241 ctgctgaagc agtactttga ctgctataat gagctgaagg ccttcaagaa ggactgtaag
      301 agcgatgagg aggaggtgaa gaaaaccgcc ctgcgcaaca agtgtacctc catccagagg
      361 gccatgcgcg aggccatctc tcaggccttt ctgaagagcc cccagaagaa gctgctggcc
      421 atcaagaacc tgatcgagaa cgtgttcaag gccgacgaga atgtgcagca cttctccgag
      481 tttaccagct atttctccgg ctttgagaca aacagagaga atttctactc tgacgaggag
      541 aagtccacat ctatcgccta taggctggtg cacgataacc tgcctatctt catcaagaac
      601 atctacatct tcgagaagct gaaggagcag ttcgacgcca agaccctgag cgagatcttc
      661 gagaactaca agctgtatgt ggccggctct agcctggatg aggtgttctc cctggagtac
      721 tttaacaata ccctgacaca gaagggcatc gacaactata atgccgtgat cggcaagatc
      781 gtgaaggagg ataagcagga gatccagggc ctgaacgagc acatcaacct gtataatcag
      841 aagcacaagg accggagact gcccttcttt atctccctga agaagcagat cctgtccgat
      901 cgggaggccc tgtcttggct gcctgacatg ttcaagaatg attctgaagt gatcaaggcc
      961 ctgaagggct tctacatcga ggacggcttt gagaacaatg tgctgacacc tctggccacc
     1021 ctgctgtcct ctctggataa gtacaacctg aatggcatct ttatccgcaa caatgaggcc
     1081 ctgagctccc tgtcccagaa cgtgtatcgg aatttttcta tcgacgaggc catcgatgcc
     1141 aacgccgagc tgcagacctt caacaattac gagctgatcg ccaatgccct gcgcgccaag
     1201 atcaagaagg agacaaagca gggccggaag tctttcgaga agtacgagga gtatatcgat
     1261 aagaaggtga aggccatcga cagcctgtcc atccaggaga tcaacgagct ggtggagaat
     1321 tacgtgagcg agtttaactc taatagcggc aacatgccaa gaaaggtgga ggactacttc
     1381 agcctgatga ggaagggcga cttcggctcc aacgatctga tcgaaaatat caagaccaag
     1441 ctgagcgccg cagagaagct gctgggcaca aagtaccagg agacagccaa ggacatcttc
     1501 aagaaggatg agaactccaa gctgatcaag gagctgctgg acgccaccaa gcagttccag
     1561 cactttatca agccactgct gggcacaggc gaggaggcag atcgggacct ggtgttctac
     1621 ggcgattttc tgcccctgta tgagaagttt gaggagctga ccctgctgta taacaaggtg
     1681 cggaatagac tgacacagaa gccctattcc aaggacaaga tccgcctgtg cttcaacaag
     1741 cctaagctga tgacaggctg ggtggattcc aagaccgaga agtctgacaa cggcacacag
     1801 tacggcggct atctgtttcg gaagaagaat gagatcggcg agtacgatta ttttctgggc
     1861 atctctagca aggcccagct gttcagaaag aacgaggccg tgatcggcga ctacgagagg
     1921 ctggattact atcagccaaa ggccaatacc atctacggct ctgcctatga gggcgagaac
     1981 agctacaagg aggacaagaa gcggctgaac aaagtgatca tcgcctatat cgagcagatc
     2041 aagcagacaa acatcaagaa gtctatcatc gagtccatct ctaagtatcc taatatcagc
     2101 gacgatgaca aggtgacccc atcctctctg ctggagaaga tcaagaaggt gtctatcgac
     2161 agctacaacg gcatcctgtc cttcaagtct tttcagagcg tgaacaagga agtgatcgat
     2221 aacctgctga aaaccatcag ccccctgaag aacaaggccg agtttctgga cctgatcaat
     2281 aaggattatc agatcttcac cgaggtgcag gccgtgatcg acgagatctg caagcagaaa
     2341 accttcatct actttccaat ctccaacgtg gagctggaga aggagatggg cgataaggac
     2401 aagcccctgt gcctgttcca gatcagcaat aaggatctgt ccttcgccaa gacctttagc
     2461 gccaacctgc ggaagaagag aggcgccgag aatctgcaca caatgctgtt taaggccctg
     2521 atggagggca accaggataa tctggacctg ggctctggcg ccatcttcta cagagccaag
     2581 agcctggacg gcaacaagcc cacacaccct gccaatgagg ccatcaagtg taggaacgtg
     2641 gccaataagg ataaggtgtc cctgttcacc tacgacatct ataagaacag gcgctacatg
     2701 gagaataagt tcctgtttca cctgagcatc gtgcagaact ataaggccgc caatgactcc
     2761 gcccagctga acagctccgc caccgagtat atcagaaagg ccgatgacct gcacatcatc
     2821 ggcatcgata ggggcgagcg caatctgctg tactattccg tgatcgatat gaagggcaac
     2881 atcgtggagc aggactctct gaatatcatc aggaacaatg acctggagac agattaccac
     2941 gacctgctgg ataagaggga gaaggagcgc aaggccaacc ggcagaattg ggaggccgtg
     3001 gagggcatca aggacctgaa gaagggctac ctgagccagg ccgtgcacca gatcgcccag
     3061 ctgatgctga agtataacgc catcatcgcc ctggaggatc tgggccagat gtttgtgacc
     3121 cgcggccaga agatcgagaa ggccgtgtac cagcagttcg agaagagcct ggtggataag
     3181 ctgtcctacc tggtggacaa gaagcggcct tataatgagc tgggcggcat cctgaaggcc
     3241 taccagctgg cctctagcat caccaagaac aattctgaca agcagaacgg cttcctgttt
     3301 tatgtgccag cctggaatac aagcaagatc gatcccgtga ccggctttac agacctgctg
     3361 cggcccaagg ccatgaccat caaggaggcc caggacttct ttggcgcctt cgataacatc
     3421 tcttacaatg acaagggcta tttcgagttt gagacaaact acgacaagtt taagatcaga
     3481 atgaagagcg cccagaccag gtggacaatc tgcaccttcg gcaatcggat caagagaaag
     3541 aaggataaga actactggaa ttatgaggag gtggagctga ccgaggagtt caagaagctg
     3601 tttaaggaca gcaacatcga ttacgagaac tgtaatctga aggaggagat ccagaacaag
     3661 gacaatcgca agttctttga tgacctgatc aagctgctgc agctgacact gcagatgcgg
     3721 aactccgatg acaagggcaa tgattatatc atctctcctg tggccaacgc cgagggccag
     3781 ttctttgact cccgcaatgg cgataagaag ctgccactgg atgcagacgc aaacggagcc
     3841 tacaatatcg cccgcaaggg cctgtggaac atccggcaga tcaagcagac caagaacgac
     3901 aagaagctga atctgagcat ctcctctaca gagtggctgg atttcgtgcg ggagaagcct
     3961 tacctgaaga aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga
     4021 tcctacccat acgatgttcc agattacgct tatccctacg acgtgcctga ttatgcatac
     4081 ccatatgatg tccccgacta tgcctaa
//
