LOCUS       sequence-124376-        3876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized PmCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY019 (pcDNA3.1-hPmCpf1)
FEATURES             Location/Qualifiers
     source          1..3876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3739..3786
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3793..3873
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atgaaaaccc agcacttctt tgaggacttc acaagcctgt actctctgag caagaccatc
       61 cggtttgagc tgaagccaat cggcaagacc ctggagaaca tcaagaagaa tggcctgatc
      121 cggagagatg agcagagact ggacgattac gagaagctga agaaagtgat cgacgagtat
      181 cacgaggatt tcatcgccaa catcctgagc tccttttcct tctctgagga gatcctgcag
      241 tcctacatcc agaatctgag cgagtccgag gccagggcca agatcgagaa aaccatgcgc
      301 gacacactgg ccaaggcctt ctctgaggat gagaggtaca agagcatctt taagaaggag
      361 ctggtgaaga aggacatccc cgtgtggtgc cctgcctata agagcctgtg caagaagttc
      421 gataacttta ccacatctct ggtgcccttc cacgagaaca ggaagaacct gtataccagc
      481 aatgagatca cagcctctat cccttatcgc atcgtgcacg tgaacctgcc aaagtttatc
      541 cagaatatcg aggccctgtg cgagctgcag aagaagatgg gcgccgacct gtacctggag
      601 atgatggaga acctgcgcaa cgtgtggccc agcttcgtga aaaccccaga cgacctgtgc
      661 aacctgaaaa cctataatca cctgatggtg cagtctagca tcagcgagta caacaggttt
      721 gtgggcggct attccaccga ggacggcaca aagcaccagg gcatcaacga gtggatcaat
      781 atctacagac agaggaataa ggagatgcgc ctgcctggcc tggtgttcct gcacaagcag
      841 atcctggcca aggtggactc ctctagcttc atcagcgata cactggagaa cgacgatcag
      901 gtgttttgcg tgctgagaca gttcaggaag ctgttttgga ataccgtgtc ctctaaggag
      961 gacgatgccg cctccctgaa ggacctgttc tgtggcctgt ctggctatga ccctgaggcc
     1021 atctacgtga gcgatgccca cctggccaca atctccaaga acatctttga cagatggaat
     1081 tacatctccg atgccatcag gcgcaagacc gaggtgctga tgccacggaa gaaggagagc
     1141 gtggagagat atgccgagaa gatctccaag cagatcaaga agagacagtc ttacagcctg
     1201 gccgagctgg acgatctgct ggcccactat agcgaggagt ccctgcccgc aggcttctct
     1261 ctgctgagct actttacatc tctgggcggc cagaagtatc tggtgagcga cggcgaagtg
     1321 atcctgtacg aggagggcag caacatctgg gacgaggtgc tgatcgcctt cagggatctg
     1381 caggtcatcc tggacaagga cttcaccgag aagaagctgg gcaaggatga ggaggccgtg
     1441 tctgtgatca agaaggccct ggacagcgcc ctgcgcctgc ggaagttctt tgatctgctg
     1501 tccggcacag gcgcagagat caggagagac agctccttct atgccctgta taccgaccgg
     1561 atggataagc tgaagggcct gctgaagatg tatgataagg tgagaaacta cctgaccaag
     1621 aagccttatt ccatcgagaa gttcaagctg cactttgaca acccatccct gctgtctggc
     1681 tgggataaga ataaggagct gaacaatctg tctgtgatct tccggcagaa cggctactat
     1741 tacctgggca tcatgacacc caagggcaag aatctgttca agaccctgcc taagctgggc
     1801 gccgaggaga tgttttatga gaagatggag tacaagcaga tcgccgagcc tatgctgatg
     1861 ctgccaaagg tgttctttcc caagaaaacc aagccagcct tcgccccaga ccagagcgtg
     1921 gtggatatct acaacaagaa aaccttcaag acaggccaga agggctttaa taagaaggac
     1981 ctgtaccggc tgatcgactt ctacaaggag gccctgacag tgcacgagtg gaagctgttt
     2041 aacttctcct tttctccaac cgagcagtat cggaatatcg gcgagttctt tgacgaggtg
     2101 agagagcagg cctacaaggt gtccatggtg aacgtgcccg cctcttatat cgacgaggcc
     2161 gtggagaacg gcaagctgta tctgttccag atctacaata aggacttcag cccctactcc
     2221 aagggcatcc ctaacctgca cacactgtat tggaaggccc tgttcagcga gcagaatcag
     2281 agccgggtgt ataagctgtg cggaggagga gagctgtttt atagaaaggc cagcctgcac
     2341 atgcaggaca ccacagtgca ccccaagggc atctctatcc acaagaagaa cctgaataag
     2401 aagggcgaga caagcctgtt caactacgac ctggtgaagg ataagaggtt taccgaggac
     2461 aagttctttt tccacgtgcc tatctctatc aactacaaga ataagaagat caccaacgtg
     2521 aatcagatgg tgcgcgatta tatcgcccag aacgacgatc tgcagatcat cggcatcgac
     2581 cgcggcgagc ggaatctgct gtatatcagc cggatcgata caaggggcaa cctgctggag
     2641 cagttcagcc tgaatgtgat cgagtccgac aagggcgatc tgagaaccga ctatcagaag
     2701 atcctgggcg atcgcgagca ggagcggctg aggcgccggc aggagtggaa gtctatcgag
     2761 agcatcaagg acctgaagga tggctacatg agccaggtgg tgcacaagat ctgtaacatg
     2821 gtggtggagc acaaggccat cgtggtgctg gagaacctga atctgagctt catgaagggc
     2881 aggaagaagg tggagaagtc cgtgtacgag aagtttgagc gcatgctggt ggacaagctg
     2941 aactatctgg tggtggataa gaagaacctg tccaatgagc caggaggcct gtatgcagca
     3001 taccagctga ccaatccact gttctctttt gaggagctgc acagataccc ccagagcggc
     3061 atcctgtttt tcgtggaccc atggaacacc tctctgacag atcccagcac aggcttcgtg
     3121 aatctgctgg gcagaatcaa ctacaccaat gtgggcgacg cccgcaagtt tttcgatcgg
     3181 tttaacgcca tcagatatga cggcaagggc aatatcctgt tcgacctgga tctgtccaga
     3241 tttgatgtga gggtggagac acagaggaag ctgtggacac tgaccacatt cggctctcgc
     3301 atcgccaaat ccaagaagtc tggcaagtgg atggtggagc ggatcgagaa cctgagcctg
     3361 tgctttctgg agctgttcga gcagtttaat atcggctaca gagtggagaa ggacctgaag
     3421 aaggccatcc tgagccagga taggaaggag ttctatgtgc gcctgatcta cctgtttaac
     3481 ctgatgatgc agatccggaa cagcgacggc gaggaggatt atatcctgtc tcccgccctg
     3541 aacgagaaga atctgcagtt cgacagcagg ctgatcgagg ccaaggatct gcctgtggac
     3601 gcagatgcaa acggagcata caatgtggcc cgcaagggcc tgatggtggt gcagagaatc
     3661 aagaggggcg accacgagtc catccacagg atcggaaggg cacagtggct gagatatgtg
     3721 caggagggca tcgtggagaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag
     3781 aaaaagggat cctacccata cgatgttcca gattacgctt atccctacga cgtgcctgat
     3841 tatgcatacc catatgatgt ccccgactat gcctaa
//
