LOCUS       sequence-12438-e       12334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, 
            Baillie D, Vidal M, Dupuy D
  TITLE     An antibiotic selection marker for nematode transgenesis.
  JOURNAL   Nat Methods. 2010 Sep;7(9):721-3. doi: 10.1038/nmeth.1494. Epub 2010
            Aug 22.
  PUBMED    20729841
REFERENCE   2  (bases 1 to 12334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1494"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "721-3"
COMMENT     Sequence Label: pdestRG5273Neo
FEATURES             Location/Qualifiers
     source          1..12334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             796..1590
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(850..869)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1460..1479
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2291..2307
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2292..2308
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3972..3990)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4011..4027)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4011..4027)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4024..4046)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4035..4051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4059..4065,4066..4083,4084..4089))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4104..4125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4242..4259)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4413..5001)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4493..4512)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5172..5963,5964..6032))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5795..5814
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6033..6137)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6163..6618)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6300..6321)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6512..6531
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6745..6767
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6759..6776
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6760..6776
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6783..6802
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6783..6801
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8292..8316
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9248..9268)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     10016..10044
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(10087..10107)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(10116..10215)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        10372..10474
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             10475..11134
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10541..10560)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             11476..11781
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11660..11679
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(12171..12294)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 catttctaaa ttttagttta ataaaaaaat tttaaaataa tacgagtttt gagactaact
       61 ttcaataaaa accaaaaaat tgcttcaaaa aagagctcgc gccgatttac gataaacaca
      121 gcgcgctacg acactccgaa aaccgcgccg caccattaca ttctccgccc caccgcacgg
      181 tttgcggaag gcgatgaaat tgctgggcct aaaattttaa cccttttttt cccgtttttc
      241 aactgttttc ccgatttttt cgatattttt ttcaggtttt tagttagaaa agtatataaa
      301 aattgcatta tttgtagttt tcgctcgtaa tttgtgatag aaaacggcca atttgcctag
      361 atttgtgatt ctaccaagtg gaatttttgg gaaaatatca atattccacg atttttattc
      421 aaatcataac gtttcgaaat tatcagctta aataaaacta aaataaataa taaaatacta
      481 aaaaccctga atttctcggt tctcggttca gcttgagttc gttctataca cgttatcgat
      541 ttttgtcatt tcgtttaatc atgggttttg attcatttta aatatgaaat ttgatttatt
      601 cctcgtttcg aagttggttt gtcttgtccg gccggtaaac aaatgttatt aaaaaaaata
      661 ataaatttcg aaatttttcg taatgttccg caatcgtgtt tttccgttta tctcgtcttc
      721 ccttccttca aatatactat ttattttctc aattcacata ttttcagcca gccctgctca
      781 acaagtggaa taaaaatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
      841 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
      901 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
      961 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     1021 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     1081 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     1141 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     1201 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     1261 gatgatctgg acgaagagca tcaggggctc gcaccagccg aactgttcgc caggctcaag
     1321 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     1381 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     1441 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     1501 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     1561 ttctatcgcc ttcttgacga gttcttctga gtccaattac tcttcaacat ccctacatgc
     1621 tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt
     1681 ctttgttttt tagcttcttt taagccacct ctaacaatga aattgtgtag attcaaaaat
     1741 agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa
     1801 ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata
     1861 aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt
     1921 tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc
     1981 atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta
     2041 tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg
     2101 aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg
     2161 gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg
     2221 gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca
     2281 taactgtttc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
     2341 tagattacat caagctcaag aatttttagt ttaacctcgt ttttatgtct agggattgtt
     2401 ttcctgagcg gattgtatcc aatgcaccca cagctaaatc tgaaaattag atttcagtga
     2461 aagttttgaa ggattcatga cgtggaccca cttgaaagtc aacctctcca tatgttacat
     2521 gtcctttcaa gtaaattaca gacagttcaa aaaagcaaag aatatttagg aaaacatgag
     2581 tgggccgaag aaatacgaca ccctcaatcc gaattccgtc ataaatcaaa agttcttgaa
     2641 attttgcaaa gtactctaac attgttatta cgcgccgcgg cgtagctttt tttaaaagtt
     2701 tcagctggta gtagaaactg aatgagagaa caaattaaac tccgaattat ggaaatagac
     2761 aatagactcc gccgcccttt tgatagtgct actatgcaac gtgatttgca catataaatt
     2821 catgtagcca agttttttga tctcttaatt gcaagtttca aaattttcat cgcaactaca
     2881 cgtctttgtc ttgtgtcacg agtagcgtca caaactccca tctcaaacat tactcattga
     2941 aaacgcaatc agttaagcac tagattaacc cttaatctga tccacaacct gaaagtgatc
     3001 ttcaaataaa aattcaaaag gaggtggctc gattgagcac ttgttttatg tcatatggga
     3061 ttttagtaag caaagttctt attttgcttt tatttggcgt ttttttttgg ggattttgaa
     3121 aaaaaaaacg ttttactaat tattgcaata tttacctagg gatgagaatt ttcaaacttc
     3181 cattttgttt gaaacataag tattaaaata ttattagaag gttctttctt tctgcagctg
     3241 acctgtttga gacacatttt tttattttat ttataaaaat tgatgattct tgttgccgtt
     3301 tccacaatgt cctcattcag taaacttgtg agattacaat tttatttcgt ttgtgtctcc
     3361 tgttttttaa aattcaatta ttttttaaat acataccata tgccgtttct tttcaatatt
     3421 tacatctgtc tggtaaatga tgtgtattaa tttattagtt agtcatttgt tttctttttc
     3481 attcaactat tttcattact ctcccacgtt tttccaacca atcgcgtcga cggaaatgta
     3541 aaatgtgaga tattgtgcgg cttcttcttt taaattcgcc ataatttatt tcactctttc
     3601 ggtcacacga ttttttttcc acaattggca ccttaaattt gacgaggcta agaaaccaaa
     3661 ggtcctcctg ctttccggtg actcgcttat agtatctgag tgctagtaaa caaagttctt
     3721 ggaacttgtg taaagcttaa tgaaggatcc agaatatgtg attactgcct gcttctaatt
     3781 acatagagga tatgatgcgc aaatttttga aagagcgata gttcagttta gtttcggctg
     3841 tgagtggcag cgcatctttt attgcttcga ttgttttgtt cctcatctca tttcttacaa
     3901 agtccagata tttagagtga cgatgcccat catccgcggc cgccaccgcg gtggagctcc
     3961 agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg
     4021 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4081 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4141 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     4201 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4261 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4321 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4381 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4441 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4501 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4561 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4621 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4681 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4741 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4801 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     4861 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4921 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4981 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5041 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5101 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5161 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5221 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5281 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5341 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5401 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5461 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5521 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5581 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5641 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5701 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5761 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5821 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5881 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5941 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6001 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6061 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6121 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     6181 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     6241 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     6301 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     6361 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     6421 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     6481 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     6541 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     6601 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     6661 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     6721 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     6781 tgtaatacga ctcactatag ggcgaattgg gtaccgtatt ataattccaa aaacacagtt
     6841 tatttgaaat atgcgcataa aaccttcgtt agcatagtcc atgacggaac ataatgcgcg
     6901 aatcgcgaaa ataattgttt gcaatgcatt gtgcctcatt tcgttattta catatgtgtg
     6961 catcatgtcc ttggttcctg ccgattacat tgtaaatgaa ctgtgaaagt acagtgtact
     7021 ttgtcctaga aggacacgtt ctttctcata ttcgtggatt tattcgtgaa attgaaaact
     7081 gacattcaca tatgtttttt catgagaata gaagttgcaa ggttccctgg atattcagta
     7141 attacatttt taccaatgtc acacactaag gggattaaga aggtcggtat gagggtgggt
     7201 tgggtggcaa acgcagtacg attaatctat gtaccttttc gctttctcat caacaaaatg
     7261 ggcattctct tcgaatttac ataatttcaa aattactttt gtttttaata taatccttcg
     7321 aatacattac accattttgc aataagtcat agatcatctt ttttgagaaa cctcgctttt
     7381 cgttaaaact atacatttaa acaaaactct ctttaataga gagcaataat gaggtgacgt
     7441 ttaaatgagt tttttgtatt aatttttagc ggaaaggaga aggaacttgt gaaaaaaaaa
     7501 ttgaaaagga acgggtttag tctgccagaa ttcctgagta ttgtcagtct gactttatat
     7561 tttgagctca ggttcatagt ttttggtttc taaattagaa ctagcacagg gcagcttact
     7621 acatattagt tttgaacttt tattttaatg attatgtatt atattcccac acttttaaat
     7681 aaaaatttta tagttataaa aatgaaagcg tgaggaatat ttatgtattt ttaggcgggg
     7741 atataataca caagtatatt tttcaaaaat atattttcat cttaatcttt aaaataggaa
     7801 gtgtaagcaa ttttatgatc tgacaactaa caggattcac gcagcactat caaaaccttc
     7861 acagggcgca aaacagctgc gatacaaacc gtatttcttc tattagtatg gttgcaatac
     7921 tatttttcga aggtcttccc gtatgcaata ctaataggga gtccaagtct aataaggagg
     7981 ccataatgaa gaaatacggt gtatagtacc gcgcgaaacc cggtagacgt cgggccacga
     8041 tagtaattac cctttcaact ttaaagactt gtcagaaaat gtggatttaa ctttaacagc
     8101 aatatcagta acgtccctca gattttcaat tttcaataat ttttattcct gtctattttt
     8161 cgccttttct aaataggaat agttaatgaa aatgttcctg cttaaaattg tatgataatc
     8221 attattttgc ctcgtcactc aattcgacgg gcccaaagct ggtaccggtc cggaattccc
     8281 gggtcgagat atcaactttg tacaagaaag ctgggttacg gccgactagt aggaaacagt
     8341 tatgtttggt atattgggaa tgtattctgt catttaaggc aaaaacccca tagacactac
     8401 tccactttca aattatcaac ttctactcac cttccactga gcctcaaacc caaaccttct
     8461 tccgatcttt cttgcatcgt gctcatcaat acttgtgatt ttagcaagaa aaacgccgtc
     8521 ctcgaaactc ttgacaaaca ataggggaaa cccccaaaaa gcaaaagcag gaaaattaat
     8581 ttcataaact ttcacttgat tgaaaaattc caaaaatact ccaaaacttt ttcacgatga
     8641 gcatgatttg acgtcatgag aggcccagac gtgcgaagaa ataaaaattg cggtcataaa
     8701 ctgaaacgta acatatgata aggtattttg tgtgcggttt tttctatgat gtttcaaaaa
     8761 aaatttatca gaagtaaaaa aaacataaga agtgtacaca gaacattgtg tagattttgg
     8821 gatagaatta ttattaatgg ggggagggag cacaattttt ttcgactttt tattacgaat
     8881 taattctatt tttgaatcta cacaatttca ttgttagagg tgacttaaaa gaagctaaaa
     8941 aacaaagaaa ttaagaagaa gtttttttga taataacaaa aatagggggt gggagcacag
     9001 ggagaaagag catgtaggga tgttgaagag taattggact tagaagtcag aggcacgggc
     9061 gcgagatgct gaaaatttaa atattactcg ttagttagta gaactcagtt taaacttact
     9121 ctgatgacag cggcccctat tatttttgac accagacaag ttggtaatgg tagcgaccgg
     9181 cgctcagttg gaattctacg aatgctattt gtatagttca tccatgccat gtgtaatccc
     9241 agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga
     9301 aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat
     9361 tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct
     9421 aattttgaag ttctgaaaat ttaaatcaga ttagttagtt agtaaaatca tgtttaaact
     9481 tacaactttg attccattct tttgtttgtc tgccatgatg tatacattgt gtgagttata
     9541 gttgtattcc aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccttttaa
     9601 ctcgattcta ttaacaaggg tatcaccttc aaacttgact tcagcacctg aaaatttaaa
     9661 tatgtatggt tagttagtac cgaactgttt aaacttacgt gtcttgtagt tcccgtcatc
     9721 tttgaaaaat atagttcttt cctgtacata accttcgggc atggcactct tgaaaaagtc
     9781 atgccgtttc atatgatctg ggtatctcga gaagcattga acaccataac agaaagtagt
     9841 gacaagtgtt ggctgaaaat ttaaataatc agggttagtt agtatatata tgtttaaact
     9901 tacccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta
     9961 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
    10021 taattcaaca agaattggga caactccagt gaaaagttct tctcctttac tcattttttc
    10081 taccggtacc ttacgcttct tctttggagc agtcaagcct gcttttttgt acaaagttgg
    10141 cattataaaa aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
    10201 taaaatcatt atttggagct ctagagcgtc gactaagttg gcagcatcac ccgacgcact
    10261 ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt
    10321 ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca
    10381 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
    10441 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
    10501 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
    10561 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
    10621 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
    10681 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
    10741 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
    10801 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
    10861 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
    10921 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
    10981 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
    11041 tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca
    11101 acagtactgc gatgagtggc agggcggggc gtaatcgcgt ggatccggct tactaaaagc
    11161 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
    11221 gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt
    11281 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
    11341 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
    11401 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
    11461 acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
    11521 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
    11581 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
    11641 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
    11701 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
    11761 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
    11821 tacagtagaa attacagaaa ctttatcacg tttagtaagt atagaggctg aaaatccaga
    11881 tgaagccgaa cgacttgtaa gagaaaagta taagagttgt gaaattgttc ttgatgcaga
    11941 tgattttcag gactatgaca ctagcgtata tgaataggta gatgttttta ttttgtcaca
    12001 caaaaaagag gctcgcacct ctttttctta tttcttttta tgatttaata cggcattgag
    12061 gacaatagcg agtaggctgg atacgacgat tccgtttgag aagaacattt ggaaggctgt
    12121 cggtcgagct cgaattctac aggtcactaa taccatctaa gtagttgatt catagtgact
    12181 gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
    12241 tattgatatt tatatcattt tacgtttctc gttcaacttt tctatacaaa gttgatatca
    12301 ctagtcggcg gccgctctag aggatccaag ggcc
//
