LOCUS       sequence-124382-         607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of nucleotide free RhoA in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Garcia-Mata R, Wennerberg K, Arthur WT, Noren NK, Ellerbroek SM, 
            Burridge K
  TITLE     Analysis of activated GAPs and GEFs in cell lysates.
  JOURNAL   Methods Enzymol. 2006;406:425-37.
  PUBMED    16472675
REFERENCE   2  (bases 1 to 607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2006"; volume: "406"; pages: "425-37"
COMMENT     Sequence Label: pGEX-4T1-RhoA G17A
FEATURES             Location/Qualifiers
     source          1..607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(469..486)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(513..535)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 agcgccaatc ctgtttgcca tatctctgcc ttcttcaggt ttcaccggct cctgcttcat
       61 cttggctagc tcccgccttg tgtgctcatc attccgaaga tccttcttat tcccaaccag
      121 gatgatgggc acgttgggac agaaatgctt gacttctggg gtccactttt ctgggatgtt
      181 ttctaaacta tcagggctgt cgatggaaaa acacatcagt ataacatcgg tatctgggta
      241 ggagaggggc ctcaggcgat cataatcttc ctgcccagct gtgtcccaca aagccaactc
      301 tacctgcttt ccatccacct cgatatctgc cacatagttc tcaaacactg tgggcacata
      361 cacctctggg aactggtcct tgctgaagac tatgagcaag catgtctttg cacaggctcc
      421 atcaccaaca atcaccagtt tcttccggat ggcagccatg aattccgggg atccacgcgg
      481 aaccagatcc gattttggag gatggtcgcc accaccaaac gtggcttgcc agccctgcaa
      541 aggccatgct atatacttgc tggatttcaa gtacttatca atttgtggga tagcttcaat
      601 acgtttt
//
