LOCUS       sequence-124398-         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral Gateway destination vector. Puromycin 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pLX301
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(104..228)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(324..344)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
       61 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
      121 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
      181 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttt agtaatgagc
      241 tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
      301 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
      361 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
      421 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
      481 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
      541 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
      601 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
      661 gggaaatcat cgtc
//
