LOCUS       sequence-124399-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-His tagged nonglycosylated human serum transferrin in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Mason AB, He QY, Halbrooks PJ, Everse SJ, Gumerov DR, Kaltashov IA, 
            Smith VC, Hewitt J, MacGillivray RT
  TITLE     Differential effect of a his tag at the N- and C-termini: functional
            studies with recombinant human serum transferrin.
  JOURNAL   Biochemistry. 2002 Jul 30;41(30):9448-54.
  PUBMED    12135367
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2002-07-30"; volume: "41(30)"; pages: 
            "9448-54"
COMMENT     Sequence Label: pNUT N6His hTFNG
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..84
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..96
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aggctcgccg tgggagccct gctggtctgc gccgtcctag ggctgtgtct ggctgtccct
       61 gataaacatc atcatcatca tcatatcgag ggaagggtcc ctgataaaac tgtgagatgg
      121 tgtgcagtgt cggagcatga ggccactaag tgccagagtt tccgcgacca tatgaaaagc
      181 gtcattccat ccgatggtcc cagtgttgct tgtgtgaaga aagcctccta ccttgattgc
      241 atcagggcca ttgcggcaaa cgaagcggat gctgtgacac tggatgcagg tttggtgtat
      301 gatgcttacc tggctcccaa taacctgaag cctgtggtgg cagagttcta tgggtcaaaa
      361 gaggatccac agactttcta ttatgctgtt gctgtggtga agaaggatag tggcttccag
      421 atgaaccagc ttcgaggcaa gaagtcctgc cacacgggtc taggcaggtc cgctgggtgg
      481 aacatcccca taggcttact ttactgtgac ttacctgagc cacgtaaacc tcttgagaaa
      541 gcagtggcca atttcttctc gggcagctgt gccccttgtg cggatgggac ggacttcccc
      601 cagctgtgtc aactgtgtcc agggtgtggc tgctccaccc ttaaccaata cttcggctac
      661 tcgggagcct tcaagtgtct gaaggatggt gctggggatg tggcctttgt caagcactcg
//
