LOCUS       sequence-124406-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IMCE attB donor copy control fosmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  TITLE     IMCE attB donor plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJH145
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(107..478)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            511..620
                     /note="incP origin of transfer"
ORIGIN
        1 agtgatgtac ttcaccagct ccgcgaagtc gctcttcttg atggagcgca tggggacgtg
       61 cttggcaatc acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat ggctctgccc
      121 tcgggcggac cacgcccatc atgaccttgc caagctcgtc ctgcttctct tcgatcttcg
      181 ccagcagggc gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg tcggtgagcc
      241 agagtttcag caggccgccc aggcggccca ggtcgccatt gatgcgggcc agctcgcgga
      301 cgtgctcata gtccacgacg cccgtgattt tgtagccctg gccgacggcc agcaggtagg
      361 ccgacaggct catgccggcc gccgccgcct tttcctcaat cgctcttcgt tcgtctggaa
      421 ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca tcagccatcc
      481 gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt
      541 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
      601 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa
      661 ccctttggca aaatcctgta tatcgtgcg
//
