LOCUS       sequence-124411-        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the N-terminal histidine-tagged DXH36 gene product upon 
            transfection into Rosetta 2 E. coli and induction with IPTG..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Vaughn JP, Creacy SD, Routh ED, Joyner-Butt C, Jenkins GS, Pauli S, 
            Nagamine Y, Akman SA
  TITLE     The DEXH protein product of the DHX36 gene is the major source of 
            tetramolecular quadruplex G4-DNA resolving activity in HeLa cell 
            lysates.
  JOURNAL   J Biol Chem. 2005 Nov 18;280(46):38117-20. Epub 2005 Sep 7.
  PUBMED    16150737
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-11-18"; volume: "280(46)"; pages: "38117-20. 
            Epub 2005 Sep 7"
COMMENT     Sequence Label: TriEx-4 DHX36
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    223..256
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_recomb     265..970
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             265..853
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     3'UTR           510..882
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     terminator      complement(983..1030)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      181 cgctcgccgc agccgaacga ccgagcgcag cgttccgagc ttataacttc gtataatgta
      241 tgctatacga agttatgcga tcgcaattcc aaaaagctca actacaaatt tgatcgcgga
      301 cgtgttagcc gacacaatta ataggcgtcg tgtggctatg gcaaaatcgt cttcggaagc
      361 aacttctaac gacgagggtt gggacgacga cgataatcgg cctaataaag ctaacacgcc
      421 cgatgttaaa tatgtccaag ctctatttaa cgtgtttacg tcgagtcaat tgtacactaa
      481 cgacagtgat gaaagaaata caaaagcgca taatattttg aacgacgtcg aacctttatt
      541 acaaaacaaa acacaaacga atatcgacaa agctagattg ctgctacaag atttggcaag
      601 ttttgtggcg ttgagcgaaa atccattaga tagtccagcc atcggttcgg aaaaacaacc
      661 cttgtttgaa actaatcgaa acctatttta caaatctatt gaggatttaa tatttaaatt
      721 cagatataaa gacgctgaaa atcatttgat tttcgctcta acataccacc ctaaagatta
      781 taaatttaat gaattattaa aatacgtaca acaattgtct gtaaatcaac aacgcacaga
      841 atctagcgct taataaatgt actaataaca atgtatcgtg ttttaatacg ccggaccagt
      901 gaacagaggt gcgtctggtg caaactcctt tactttgaac accagggaaa cttcaaggag
      961 aatttcctcc gcatgctttc agcaaaaaac ccctcaagac ccgtttagag gccccaaggg
     1021 gttatgctag ttacatatgg gcatatgttg ccaaactcta
//
