LOCUS       sequence-124417-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral backbone for cloning and expression of new shRNA 
            sequences. Uses puromycin for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     Sequence Label: pLKO.1 puro
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    78..195
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        244..754
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     673..691
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(776..795)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 aaaggacgaa acaccggtcc gcaggtatgc acgcgtgaat tctcgacctc gagacaaatg
       61 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
      121 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
      181 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc
      241 gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
      301 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
      361 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
      421 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
      481 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
      541 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
      601 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
      661 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
      721 cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac
      781 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccag
//
