LOCUS       sequence-124422-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  post.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Kim J, Zhao T, Petralia RS, Yu Y, Peng H, Myers E, Magee JC
  TITLE     mGRASP enables mapping mammalian synaptic connectivity with light 
            microscopy.
  JOURNAL   Nat Methods. 2011 Dec 4;9(1):96-102. doi: 10.1038/nmeth.1784.
  PUBMED    22138823
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-4- 4"; volume: "9"; 
            issue: "1"; pages: "96-102"
COMMENT     Sequence Label: paavCAG-post-mGRASP-2A-dTomato
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     90..107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    315..436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(352..371)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     406..425
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             585..596
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
       61 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgagcga gcgcgcagag
      121 agggagtggc caactccatc actaggggtt ccttgtagtt aatgattaac ccgccatgct
      181 acttatctac gtagccatgc tctaggaaga tccttatcga ttttaccaca tttgtagagg
      241 ttttacttgc tttaaaaaac ctcccacatc tccccctgaa cctgaaacat aaaatgaatg
      301 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      361 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      421 tcatcaatgt atcttatcat gtctgctcga agcggccgcc cgggtcgacg gtatcgatgc
      481 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
      541 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
      601 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga
      661 aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
      721 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gaggg
//
