LOCUS       sequence-124431-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with pco-dCas9-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ152
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             115..162
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             169..189
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtac aaaaaagcag gctccgaatt cgcccttcac catggattac
      121 aaggatgatg atgataagga ttacaaggat gatgatgata agatggctcc aaagaagaag
      181 agaaaggttg gaatccacgg agttccagct gctgataaga agtactctat cggacttgcc
      241 atcggaacca actctgttgg atgggctgtt atcaccgatg agtacaaggt tccatctaag
      301 aagttcaagg ttcttggaaa caccgataga cactctatca agaagaacct tatcggtgct
      361 cttcttttcg attctggaga gaccgctgag gctaccagat tgaagagaac cgctagaaga
      421 agatacacca gaagaaagaa cagaatctgc taccttcagg aaatcttctc taacgagatg
      481 gctaaggttg atgattcttt cttccacaga cttgaggagt ctttccttgt tgaggaggat
      541 aagaagcacg agagacaccc aatcttcgga aacatcgttg atgaggttgc ttaccacgag
      601 aagtacccaa ccatctacca ccttagaaag aagttggttg attctaccga taaggctgat
      661 cttagactta tctaccttgc tcttgctcac atgatcaagt tcagaggaca cttccttatc
      721 gagggagacc ttaacccaga taactctgat gttgataagt tgttcatcca gcttgttcag
      781 acctacaacc agcttttcga ggagaaccca atcaacgctt ctggagttga tgctaaggct
      841 atcctttctg ctagactttc taagtctcgt agacttgaga accttatcgc tcagcttcca
      901 ggagagaaga agaacggact tttcggaaac cttatcgctc tttctcttgg acttacccca
      961 aacttcaagt ctaacttcga tcttgctgag gatgctaagt tgcagctttc taaggatacc
     1021 t
//
