LOCUS       sequence-124432-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with pco-dCas9-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ152
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(14..137)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(182..434)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(619..666)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 atcagctgga tggcatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
       61 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac
      121 ttttgtatac aaagttgcga attcgccctt tccggaagct gtcgaggggg gatcaattcc
      181 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
      241 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
      301 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
      361 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
      421 atgtttgaac gatcggacgt cctaaagcat gtcgaggtcg aagtcatcga gtgcgtcgag
      481 catgtcaagg tcaaagtcat caagtgcatc gagcatgtcg agatcaaaat catcaagagc
      541 gtcgagcata tcgaggtcaa aatcgtcaag agcatcgtcg actgagccag aacccgaccc
      601 cgatccagag ccgtctccct tcttcttctt agcctgtcca gccttcttgg tagcagctgg
      661 tctcttatct cctccaagct gagaaagatc gattctggtc tcgtaaagtc cggtgataga
      721 ctggtggata agggtagcat caagaacctc cttggtagag gtgtatcttt ttctatcgat
      781 ggtggtatcg aagtacttga aagcagctgg agcaccaagg ttggtaaggg tgaaaaggtg
      841 gatgatgttc tcagcctgct ctctgattgg cttatctctg tgcttgttgt aagcagaaag
      901 aaccttatca aggttagcat cagcaaggat aactctctta gagaactcag agatttgctc
      961 gatgatctca tcaaggtagt gcttgtgctg ctcaacgaaa agctgcttc
//
