LOCUS       sequence-124438-         431 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 1st gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ131A
FEATURES             Location/Qualifiers
     source          1..431
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(371..376,377..393,394..399))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagaga atccacagga cgggtgtggt cgccatgatc
       61 gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg
      121 tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc
      181 agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc
      241 agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg
      301 atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat
      361 aaactaccgc attaaagcta atcgatgata agctgtcaaa catgagaatt aattcatgtt
      421 ctttcctgcg t
//
