LOCUS       sequence-124439-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Jones SA, Shim SH, He J, Zhuang X
  TITLE     Fast, three-dimensional super-resolution imaging of live cells.
  JOURNAL   Nat Methods. 2011 Jun;8(6):499-508. Epub 2011 May 8.
  PUBMED    21552254
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-06"; volume: "8(6)"; pages: "499-508. Epub 
            2011 May 8"
COMMENT     Sequence Label: pSNAP-CLC-SNAP
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(148..693)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for mammalian expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct agagtcgcgg ccgctcaacc cagcccaggc ttgcccagtc tgtggccctc
      181 gtgggccagc agccactctt tcacggcgag cccgccctcg tagcccccca cggcgccaga
      241 gctagacacc acccggtggc aggggatcag aatgggcacg ggatttccgc tcagggcggt
      301 tttcacggcg gcggtggcgg cgggattgcc ggccagggcg gccagctgct ggtagctgat
      361 gacctctccg aacttcacca ctttcagcag tttccacagc acctggcggg taaagctctc
      421 ctgctggaac actgggtggt gcagggctgg cacagggaac tcctcgatgg cctcaggctg
      481 gtgaaagtag gcgttgagcc aggcggtggc ctgcatcagt ggctctggtc cgcccagcac
      541 ggcggctggg gcaggcactt ccacggcgtc ggcggcagat gttcctttgc ccagcagctt
      601 gatctcgtgc aggccctgtt cgcacccaga cagttccagc ttgcccagag ggctatccag
      661 ggtggtgcgc ttcatttcgc agtctttgtc catcaccggt gggtgcacca ggggggcctg
      721 cttcagggag atgaggactg agcgcatgcg agagacatct ttggcctgtt tgctggactt
      781 ggggttaaag tcacacaggc gggccacccg ctcccactca gtgcctgggg atgactc
//
