LOCUS       sequence-124440-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 3rd gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ133A
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(347..366)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(348..366)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(371..387)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(371..387)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        476..779
                     /label=AtU6-1 promoter
                     /note="promoter for the Arabidopsis thaliana U6-1 snRNA 
                     gene (Waibel and Filipowicz, 1990)"
     misc_RNA        813..888
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tcgcggctct taccagccta acttcgatca ttggaccgct gatcgtcacg gcgatttatg
       61 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg
      121 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag
      181 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg
      241 gagaactgtg aatgcgcaaa ccaacccttg atcggggaag aacagtatgt cgagctattt
      301 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatatcccct atagtgagtc
      361 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
      421 ttacattgca caagataaaa atatatcatc atgcctcctc tagaggtctc aggacagaaa
      481 tctcaaaatt ccggcagaac aattttgaat ctcgatccgt agaaaccaga cggtcattgt
      541 tttagttcca ccacgattat atttgaaatt tacgtgagtg tgagtgagac ttgcataaga
      601 aaataaaatc tttagttggg aaaaaattca ataatataaa tgggcttgag aaggaagcga
      661 gggataggcc tttttctaaa ataggcccat ttaagctatt aacaatcttc aaaagtacca
      721 cagcgcttag gtaaagaaag cagctgagtt tatatatggt tagacacgaa gtagtgattg
      781 gagacgagat ctagtctgag tcgaccgtct ctgttttaga gctagaaata gcaagttaaa
      841 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttggcaa
      901 aaattttcag attttttctt catctgtaga tttctgggtt tttttttccg tttcgtgga
//
