LOCUS       sequence-124441-         457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 3rd gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ133A
FEATURES             Location/Qualifiers
     source          1..457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(376..381,382..398,399..404))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agagaatcca caggacgggt gtggtcgcca
       61 tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa
      121 agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg
      181 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc
      241 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga
      301 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact
      361 gtgataaact accgcattaa agctaatcga tgataagctg tcaaacatga gaattaattc
      421 atgttctttc ctgcgttatc ccctgattct gtggata
//
