LOCUS       sequence-124443-        3565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone containing secreted human AnxaV with no stop 
            codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Morsch M, Radford R, Lee A, Don EK, Badrock AP, Hall TE, Cole NJ, 
            Chung R
  TITLE     In vivo characterization of microglial engulfment of dying neurons 
            in the zebrafish spinal cord.
  JOURNAL   Front Cell Neurosci. 2015 Aug 31;9:321. doi: 
            10.3389/fncel.2015.00321. eCollection 2015.
  PUBMED    26379496
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Neurosci."; date: "2015-08-31"; volume: "9:321. doi"; pages: " 
            10.3389/fncel.2015.00321. eCollection 2015"
COMMENT     Sequence Label: pME-secAnxaV-NS
FEATURES             Location/Qualifiers
     source          1..3565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      669..678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1690..1789)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1806..1825)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1807..1825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1830..1846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1830..1846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1948..1967)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1959..2768
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2026..2045)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2915..3503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3404..3423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgc caccatggtg cacaaggtgc tgctggccct gttcttcatc ttcctggccc
      721 ccgccggcac cgcacaggtt ctcagaggca ctgtgactga cttccctgga tttgatgagc
      781 gggctgatgc agaaactctt cggaaggcta tgaaaggctt gggcacagat gaggagagca
      841 tcctgactct gttgacatcc cgaagtaatg ctcagcgcca ggaaatctct gcagctttta
      901 agactctgtt tggcagggat cttctggatg acctgaaatc agaactaact ggaaaatttg
      961 aaaaattaat tgtggctctg atgaaaccct ctcggcttta tgatgcttat gaactgaaac
     1021 atgccttgaa gggagctgga acaaatgaaa aagtactgac agaaattatt gcttcaagga
     1081 cacctgaaga actgagagcc atcaaacaag tttatgaaga agaatatggc tcaagcctgg
     1141 aagatgacgt ggtgggggac acttcagggt actaccagcg gatgttggtg gttctccttc
     1201 aggctaacag agaccctgat gctggaattg atgaagctca agttgaacaa gatgctcagg
     1261 ctttatttca ggctggagaa cttaaatggg ggacagatga agaaaagttt atcaccatct
     1321 ttggaacacg aagtgtgtct catttgagaa aggtgtttga caagtacatg actatatcag
     1381 gatttcaaat tgaggaaacc attgaccgcg agacttctgg caatttagag caactactcc
     1441 ttgctgttgt gaaatctatt cgaagtatac ctgcctacct tgcagagacc ctctattatg
     1501 ctatgaaggg agctgggaca gatgatcata ccctcatcag agtcatggtt tccaggagtg
     1561 agattgatct gtttaacatc aggaaggagt ttaggaagaa ttttgccacc tctctttatt
     1621 ccatgattaa gggagataca tctggggact ataagaaagc tcttctgctg ctctgtggag
     1681 aagatgacta cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
     1741 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     1801 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     1861 tgtctcaaaa tctctgatgt tacattgcac aagataaaat aatatcatca tgaacaataa
     1921 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
     1981 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     2041 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
     2101 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     2161 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     2221 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
     2281 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     2341 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     2401 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     2461 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     2521 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     2581 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     2641 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     2701 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     2761 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     2821 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     2881 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2941 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3001 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3061 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3121 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3181 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3241 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3301 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3361 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3421 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3481 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3541 tttgctggcc ttttgctcac atgtt
//
