LOCUS       sequence-124445-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 4th gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ134A
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(348..367)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(349..367)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(372..388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(372..388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        477..780
                     /label=AtU6-1 promoter
                     /note="promoter for the Arabidopsis thaliana U6-1 snRNA 
                     gene (Waibel and Filipowicz, 1990)"
     misc_RNA        814..889
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ctcgcggctc ttaccagcct aacttcgatc attggaccgc tgatcgtcac ggcgatttat
       61 gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt
      121 gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa
      181 gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc
      241 ggagaactgt gaatgcgcaa accaaccctt gatcggggaa gaacagtatg tcgagctatt
      301 ttttgactta ctggggatca agcctgattg ggagaaaata aaatatcccc tatagtgagt
      361 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
      421 gttacattgc acaagataaa aatatatcat catgcctcct ctagaggtct cgccagagaa
      481 atctcaaaat tccggcagaa caattttgaa tctcgatccg tagaaaccag acggtcattg
      541 ttttagttcc accacgatta tatttgaaat ttacgtgagt gtgagtgaga cttgcataag
      601 aaaataaaat ctttagttgg gaaaaaattc aataatataa atgggcttga gaaggaagcg
      661 agggataggc ctttttctaa aataggccca tttaagctat taacaatctt caaaagtacc
      721 acagcgctta ggtaaagaaa gcagctgagt ttatatatgg ttagacacga agtagtgatt
      781 ggagacgaga tctagtctga gtcgaccgtc tctgttttag agctagaaat agcaagttaa
      841 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggca
      901 aaaattttca gattttttct t
//
