LOCUS       sequence-124447-         403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 4th gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ134A
FEATURES             Location/Qualifiers
     source          1..403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(273..278,279..295,296..301))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg
       61 catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac
      121 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag
      181 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact
      241 gcgttagcaa tttaactgtg ataaactacc gcattaaagc taatcgatga taagctgtca
      301 aacatgagaa ttaattcatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      361 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aac
//
