LOCUS       sequence-124451-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 6th gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ136A
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(344..363)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(345..363)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(368..384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(368..384)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        473..776
                     /label=AtU6-1 promoter
                     /note="promoter for the Arabidopsis thaliana U6-1 snRNA 
                     gene (Waibel and Filipowicz, 1990)"
ORIGIN
        1 cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg atttatgccg
       61 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
      121 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg
      181 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag
      241 aactgtgaat gcgcaaacca acccttgatc ggggaagaac agtatgtcga gctatttttt
      301 gacttactgg ggatcaagcc tgattgggag aaaataaaat atcccctata gtgagtcgta
      361 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
      421 cattgcacaa gataaaaata tatcatcatg cctcctctag aggtctcgtg caagaaatct
      481 caaaattccg gcagaacaat tttgaatctc gatccgtaga aaccagacgg tcattgtttt
      541 agttccacca cgattatatt tgaaatttac gtgagtgtga gtgagacttg cataagaaaa
      601 taaaatcttt agttgggaaa aaattcaata atataaatgg gcttgagaag gaagcgaggg
      661 ataggccttt ttctaaaata ggcccattta agctattaac aatcttcaaa agtaccacag
      721 cgcttaggta aagaaagcag ctgagtttat atatggttag acacgaagta gtgattggag
      781 acgagatcta g
//
