LOCUS       sequence-124455-         229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 1st gRNA under OsU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ131C
FEATURES             Location/Qualifiers
     source          1..229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(129..134,135..151,152..157))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat
       61 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa
      121 actaccgcat taaagctaat cgatgataag ctgtcaaaca tgagaattaa ttcatgttct
      181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttga
//
