LOCUS       sequence-124458-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of fluorescently tagged PTRF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Hill MM, Bastiani M, Luetterforst R, Kirkham M, Kirkham A, Nixon SJ,
            Walser P, Abankwa D, Oorschot VM, Martin S, Hancock JF, Parton RG
  TITLE     PTRF-Cavin, a conserved cytoplasmic protein required for caveola 
            formation and function.
  JOURNAL   Cell. 2008 Jan 11;132(1):113-24. doi: 10.1016/j.cell.2007.11.042.
  PUBMED    18191225
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2007.11"; journalName: "Cell"; date: "2008-01-11- 
            11"; volume: "132"; issue: "1"; pages: "113-24"
COMMENT     Sequence Label: pEGFP-PTRF
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(42..44,45..47,48..758)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(87..108)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(348..367)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     695..716
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg
       61 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      121 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      181 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      241 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      301 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      361 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      421 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      481 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
      541 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      601 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca
      661 aactgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      721 ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga tctcgagctc
      781 aagcttcgaa ttctatggag gatgtcacgc tccatatcgt tgagcggccg tattccggat
      841 ttcccgatgc ttcctca
//
