LOCUS       sequence-124461-        2195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in 
            the 2nd intron of gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU1
FEATURES             Location/Qualifiers
     source          1..2195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(31..59)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(399..432)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        436..483
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(1665..1681)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1665..1681)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(1690..1723)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     2132..2152
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg ggtaagttta
      181 aacatatata tactaactaa ccctgattat ttaaattttc agccaacact tgtcactact
      241 ttctgttatg gtgttcaatg cttctcgaga tacccagatc atatgaaacg gcatgacttt
      301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      361 gggaactaca agacacgtaa gtttaaacag ttcggtacga agttcctata ctttctagag
      421 aataggaact tccctgttga caattaatca tcggcatagt atatcggcat agtataatac
      481 gacaaggtga ggaactaaac ccaggaggca gatcatgagt ctgaaagaaa aaacacaatc
      541 tctgtttgcc aacgcatttg gctaccctgc cactcacacc attcaggcgc ctggccgcgt
      601 gaatttgatt ggtgaacaca ccgactacaa cgacggtttc gttctgccct gcgcgattga
      661 ttatcaaacc gtgatcagtt gtgcaccacg cgatgaccgt aaagttcgcg tgatggcagc
      721 cgattatgaa aatcagctcg acgagttttc cctcgatgcg cccattgtcg cacatgaaaa
      781 ctatcaatgg gctaactacg ttcgtggcgt ggtgaaacat ctgcaactgc gtaacaacag
      841 cttcggcggc gtggacatgg tgatcagcgg caatgtgccg cagggtgccg ggttaagttc
      901 ttccgcttca ctggaagtcg cggtcggaac cgtattgcag cagctttatc atctgccgct
      961 ggacggcgca caaatcgcgc ttaacggtca ggaagcagaa aaccagtttg taggctgtaa
     1021 ctgcgggatc atggatcagc taatttccgc gctcggcaag aaagatcatg ccttgctgat
     1081 cgattgccgc tcactgggga ccaaagcagt ttccatgccc aaaggtgtgg ctgtcgtcat
     1141 catcaacagt aacttcaaac gtaccctggt tggcagcgaa tacaacaccc gtcgtgaaca
     1201 gtgcgaaacc ggtgcgcgtt tcttccagca gccagccctg cgtgatgtca ccattgaaga
     1261 gttcaacgct gttgcgcatg aactggaccc gatcgtggca aaacgcgtgc gtcatatact
     1321 gactgaaaac gcccgcaccg ttgaagctgc cagcgcgctg gagcaaggcg acctgaaacg
     1381 tatgggcgag ttgatggcgg agtctcatgc ctctatgcgc gatgatttcg aaatcaccgt
     1441 gccgcaaatt gacactctgg tagaaatcgt caaagctgtg attggcgaca aaggtggcgt
     1501 acgcatgacc ggcggcggat ttggcggctg tatcgtcgcg ctgatcccgg aagagctggt
     1561 gcctgccgta cagcaagctg tcgctgaaca atatgaagca aaaacaggta ttaaagagac
     1621 tttttacgtt tgtaaaccat cacaaggagc aggacagtgc tgaggatcca ctagttctag
     1681 agcggccgcg aagttcctat actttctaga gaataggaac ttctaactaa ccatacatat
     1741 ttaaattttc aggtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     1801 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     1861 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttgtaa
     1921 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagaac ttcaaaatta
     1981 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     2041 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     2101 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     2161 ggattacaca tggcatggat gaactataca aaaag
//
