LOCUS       sequence-124463-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type SpCas9 fused to Zif268 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(366..392)
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             complement(405..494)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(495..515)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tatacgactc actatagttc tagaggctcg agaggatcaa ccactttgta caagaaagct
       61 gggtctagat atctcgaggc ggccgcttag tccttctgtc ttaaatggat tttggtatgc
      121 ctcttgcgtt catcactcct ggcaaacttc ctcccacaaa tgtcacaggc aaaaggcttc
      181 tcgcctgtgt gggtgcggat gtgggtggta aggtggtcac tacgactgaa gttacgcatg
      241 cagattcgac actggaaggg cttctgaccg gtatggatgc ggatatggcg ggtaagctca
      301 tccgagcgag aaaagcggcg atcgcaggac tcgacagggc aagcatatgg gcgtccggat
      361 tcgaaatcca gtttcttttt cttagctgcc ggagcggcgg atccagcgta atctggaacg
      421 tcataaggat acgatcctgc atagtccggg acgtcatagg gatagcccgc atagtcagga
      481 acatcgtatg ggtatacctt cctcttcttc ttgggcccgc cggtgccgtc tccaccgagc
      541 tgagagaggt cgattcttgt ttcatagagc cccgtaattg actgatgaat cagtgtggcg
      601 tccaggacct cctttgtaga ggtgtaccgc tttctgtcta tggtggtgtc gaagtacttg
      661 aaggctgcag gcgcgcccaa gttggtcaga gtaaacaagt ggataatgtt ttctgcctgc
      721 tccctgatgg gcttatccct gtgcttattg taagcagaaa gcaccttatc gaggttagcg
      781 tcggcgagga tcactctttt ggagaactcg cttatttgct cgatgatctc atcaaggtag
      841 tgtttgtgtt gttccacgaa cagctgcttc tgctcattat cttcgggaga ccctttgagc
      901 ttttt
//
