LOCUS       sequence-124465-         584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type SpCas9 fused to Zif268 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268
FEATURES             Location/Qualifiers
     source          1..584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..32
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggatcaaca agtttgtaca aaaaagcagg ctggcgccac catggacaag aagtactcca
       61 ttgggctcga tatcggtacc aacagcgtcg gctgggccgt cattacggac gagtacaagg
      121 tgccgagcaa aaaattcaaa gttctgggca ataccgatcg ccacagcata aagaagaacc
      181 tcattggagc cctcctgttc gactccgggg agacggccga agccacgcgg ctcaaaagaa
      241 cagcacggcg cagatatacc cgcagaaaga atcggatctg ctacctgcag gagatcttta
      301 gtaatgagat ggctaaggtg gatgactctt tcttccatag gctggaggag tcctttttgg
      361 tggaggagga taaaaagcac gagcgccacc caatctttgg caatatcgtg gacgaggtgg
      421 cgtaccatga aaagtaccca accatatatc atctgaggaa gaagctggta gacagtactg
      481 ataaggctga cttgcggttg atctatctcg cgctggcgca catgatcaaa tttcggggac
      541 acttcctcat cgagggggac ctgaacccag acaacagcga tgtc
//
