LOCUS       sequence-124469-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type SpCas9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-NLS-3XHA-NLS
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tggacaagaa gtactccatt gggctcgata tcggtaccaa cagcgtcggc tgggccgtca
       61 ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc
      121 acagcataaa gaagaacctc attggagccc tcctgttcga ctccggggag acggccgaag
      181 ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct
      241 acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc
      301 tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca
      361 atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga
      421 agctggtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcaca
      481 tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg
      541 tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgag gagaacccga
      601 tcaacgcatc cggcgttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc
      661 ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaat
//
