LOCUS       sequence-124472-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of the ""TOP1"" construct, targeting the GLuc 
            reporter, in mammalian cells. CMV/3' Box expression backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pCMV/3' Box_(GLuc)_TOP1
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(413..488)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(544..747)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(573..593)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        748..1051
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gacgccaacc aagacacaaa ccaaacaaaa aaagatatga cccttagcgt acagtctact
       61 tttgaaactc cagaaagttg aactgcatcc atatcaacag aaaaagcagc gacgccatat
      121 cgtctgctgc aaaaaggagc gagcccatat gctctgctcc aaaaaccagc gacgccatat
      181 cgtctgctgg aaaaaccacg gagcccatat gctctgctgg aaaaaggagc gacgccatat
      241 cgtctgctcc aaaaactgtt gacttaggac ttggctgcgt gttaggacca tgtccgtcag
      301 cttattacca taccctttgc aaggccatct caaagtttcc cctgagactt ggtactgaac
      361 ggctgttgac ccctttcccg caattccggg tttagaggag ggatgttgaa gagcaccgac
      421 tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc
      481 tctaaaacat agtgagtcgt atctagatcg ccagtaagca gtgggttctc tagttagcca
      541 gagagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
      601 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
      661 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
      721 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
      781 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
      841 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
      901 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
      961 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     1021 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc ata
//
