LOCUS       sequence-124474-         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type SpCas9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-NLS-3XHA-NLS
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(100..126)
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             complement(139..228)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(229..249)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcaacc actttgtaca agaaagctgg
       61 gtctagatat ctcgaggcgg ccgcttatcc ggattcgaaa tccagtttct ttttcttagc
      121 tgccggagcg gcggatccag cgtaatctgg aacgtcataa ggatacgatc ctgcatagtc
      181 cgggacgtca tagggatagc ccgcatagtc aggaacatcg tatgggtata ccttcctctt
      241 cttcttgggc ccgccggtgc cgtctccacc gagctgagag aggtcgattc ttgtttcata
      301 gagccccgta attgactgat gaatcagtgt ggcgtccagg acctcctttg tagaggtgta
      361 ccgctttctg tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc ccaagttggt
      421 cagagtaaac aagtggataa tgttttctgc ctgctccctg atgggcttat ccctgtgctt
      481 attgtaagca gaaagcacct tatcgaggtt agcgtcggcg aggatcactc ttttggagaa
      541 ctcgcttatt tgctcgatga tctcatcaag gtagtgtttg tgttgttcca cgaacagctg
      601 cttctgctca ttatcttcgg gagacccttt gagcttttca tagtggctgg ccagatacaa
      661 gaaattaacg tatttagagg gc
//
