LOCUS       sequence-124479-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-Display Cypridina Luciferase-2A-Venus YFP ""normalizer,"" 
            transient format..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pTrans_9xD3B_Cluc_Norm
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..82)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gctcctcgcc cttgctcacc atactgcctg ggccaggatt ctcctcgacg tcaccgcatg
       61 ttagcagact tcctctgccc tctccactgc cgccgctggt gctgcctttg cattcatctg
      121 gtacttctag ggtgtctcca tgctttatgt agcattcttt cttgaactcc catgcgtggt
      181 cacagaaacc ctgttgtccc ctcaggcaat actcgtacat gcatcgttcg acacggtcag
      241 gcttgtggca cacgttacat ttctgatcaa gatcactttg accggcgaag agactattgc
      301 agagtcgttc agcttcaggt ttctgttctt cggtgcatcc cggtgggttg ggggtcagat
      361 cacaggctcc ttcagcatca aaagaatcat cactgaaatc ctggttgtag ttaccgcaaa
      421 taccgcaagt cttaccatcg aatggatctc taatatgtac gacgagcagt tgcttgaagt
      481 tgaacttgac caccagagct tcaggtagga tggctgtagt cagtatgtca ccatcttgcc
      541 agtagatgga agtgttctga gagctgtacg ggacggacac ggcttctcct ccaaccagaa
      601 tctgttttcc atcaacaatg agttctacta cagtcgattg tttcctgatt cgtactttct
      661 ctacttcagt gtaagagtca acattcctgt gtgaaacctt cacatcccaa gtgttccaga
      721 aacagtcggc ggccataaga atctccttgc agggaccctg gaattggtat cttgctttgt
      781 caaatgtatc gtagaaagta tgtccagaca aaacgcaggt tcctctagtt tcgggagcag
      841 cgcacgtctc cctgtagtca agcagcacgt gtgaggctcg ctcgtctcca cccatacagc
      901 gggcgaaggc gcaggagatg gtgtagtagt agaagttgat ggggttgcgg cagctgtcct
      961 tgtacggctc cagaagacct ttgcagtatg caacgtcatc aggatttcca tagagtggac
     1021 aaccgtcaaa ctcctggttg atcttaggct gaatagcgag ttgttctgga tctgaagtga
     1081 agtc
//
