LOCUS       sequence-124480-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multisite gateway entry clone for adding C-terminal fusions of 
            mVenus (no ATG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Morsch M, Radford R, Lee A, Don EK, Badrock AP, Hall TE, Cole NJ, 
            Chung R
  TITLE     In vivo characterization of microglial engulfment of dying neurons 
            in the zebrafish spinal cord.
  JOURNAL   Front Cell Neurosci. 2015 Aug 31;9:321. doi: 
            10.3389/fncel.2015.00321. eCollection 2015.
  PUBMED    26379496
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Neurosci."; date: "2015-08-31"; volume: "9:321. doi"; pages: " 
            10.3389/fncel.2015.00321. eCollection 2015"
COMMENT     Sequence Label: p3E-mVenus
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..114
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(119..370)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(161..182)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     510..529
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acgctaccat ggagctccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
       61 aacaaattga taagcaatgc ttttttataa tgccaacttt gtataataaa gttggttact
      121 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
      181 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctggtagctc aggtagtggt
      241 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
      301 gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct
      361 tctgcttgtc ggcggtgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
      421 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      481 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
      541 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
      601 tgtggtcggg gtagcgggcg aagcactgca ggccgtagcc cagggtggtc acgagggtgg
      661 gccagggcac gggcagcttg ccggtggtgc agatcagctt cagggtcagc ttgccgtagg
      721 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg g
//
