LOCUS       sequence-124486-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of Sp-dCas9 in mammalian cells, under an 
            EF1-alpha promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pEF_dCas9
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             49..69
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtgtccgct agagatccgc ggccgcgaga gccgccacca tggctagccc caaaaagaag
       61 aggaaagtgg ataaaaagta ttctattggt ttagccatcg gcactaattc cgttggatgg
      121 gctgtcataa ccgatgaata caaagtacct tcaaagaaat ttaaggtgtt ggggaacaca
      181 gaccgtcatt cgattaaaaa gaatcttatc ggtgccctcc tattcgatag tggcgaaacg
      241 gcagaggcga ctcgcctgaa acgaaccgct cggagaaggt atacacgtcg caagaaccga
      301 atatgttact tacaagaaat ttttagcaat gagatggcca aagttgacga ttctttcttt
      361 caccgtttgg aagagtcctt ccttgtcgaa gaggacaaga aacatgaacg gcaccccatc
      421 tttggaaaca tagtagatga ggtggcatat catgaaaagt acccaacgat ttatcacctc
      481 agaaaaaagc tagttgactc aactgataaa gcggacctga ggttaatcta cttggctctt
      541 gcccatatga taaagttccg tgggcacttt ctcattgagg gtgatctaaa tccggacaac
      601 tcggatgtcg acaaactgtt catccagtta gtacaaacct ataatcagtt gtttgaagag
      661 aaccctataa atgcaagtgg cgtggatgcg aaggctattc ttagcgcccg cctctctaaa
      721 tcccgacggc tagaaaacct gatcgcacaa ttacccggag agaagaaaaa tgggttgttc
      781 ggtaacctta tagcgctctc actaggcctg acaccaaatt ttaagtcgaa cttcgactta
      841 gctgaagatg ccaaattgca gcttagtaag gacacgtacg atgacgatct cgacaatcta
      901 ctggcacaaa ttggagatca gtatgcggac ttatttttgg ctgccaaaaa ccttagcgat
      961 gcaatcctcc tatctgacat actgagagtt aatactgaga ttaccaaggc gccgttatcc
     1021 gcttcaatga tcaaaaggta cgatgaacat caccaagact tgacacttct caaggcccta
     1081 gtccgt
//
