LOCUS       sequence-124488-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of Sp-dCas9 in mammalian cells, under an 
            EF1-alpha promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pEF_dCas9
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..105)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(112..132)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gatggtgatg atgaccggtt atccaccgcc tcctgcagcc ttgtcatcgt catccttgta
       61 atcgatgtca tgatctttat aatcaccgtc atggtctttg tagtcgctcg agactttcct
      121 cttcttcttg ggggatccgt cacccccaag ctgtgacaaa tctatccgag tttcatataa
      181 tcccgtgatg gattggtgaa tcagtgtcgc gtctagcacc tccttggtag aagtgtatcg
      241 tttgcgatct atcgttgtgt caaaatactt gaatgcggct ggagcgccga ggttggtaag
      301 agtaaacaaa tggataatat tttccgcctg ctcacgtatg ggtttatccc tgtgcttgtt
      361 gtatgcgctt aatactttgt ccagattggc atcagctagg atgactctct tactgaattc
      421 cgaaatttgc tctatgattt cgtcgagata atgtttgtgc tgctcaacaa aaagttgctt
      481 ctgttcgtta tcttcaggtg aacctttcaa cttctcgtaa tgggacgcta aatacaggaa
      541 attcacgtat ttagacggta gtgcgagttc gttccccttt tgaagctctc cggcgctagc
      601 caacatccgt tttcggccat tttctaactc aaacagacta tactttggta gtttaattat
      661 gagatccttt tttacttcct tgtaaccttt cgcctcaagg aagtcgatgg ggttcttttc
      721 aaaagacgag cgctccataa tcgttatccc caataattct ttgactgact tcagtttctt
      781 ggattttccc ttctcaactt ttgccactac taggacagaa taggcaactg tagggctatc
      841 gaagccaccg tactttttcg ggtcccagtc ctttttacga gcgatgagct tatcactatt
      901 cctttttgga agaatcgatt cctttgaaaa ccctccggtc tgcacctcag ttttctttac
      961 tatgttgact tggggcatgg acaaaacttt tctcaccgtc gcgaagtccc ggcccttatc
     1021 ccatacgatt tcacctgtct ccccattggt ttcaattaaa ggtcgtt
//
