LOCUS       sequence-124489-        1144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RFP-Atg8 (PAS  marker) with  improved  property.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: PCUP1-DuDre-Atg8-404
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        290..524
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     primer_bind     complement(788..808)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1006..1025
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac
       61 atctgggtat tactacggca aacttcaacg atttctatga tgcattttat aattagtaag
      121 ccgatcccat taccgacatt tgggcgctat acgtgcatat gttcatgtat gtatctgtat
      181 ttaaaacact tttgtattat ttttcctcat atatgtgtat aggtttatac ggatgattta
      241 attattactt caccaccctt tatttcaggc tgatatctta gccttgttac tagttagaaa
      301 aagacatttt tgctgtcagt cactgtcaag agattctttt gctggcattt cttctagaag
      361 caaaaagagc gatgcgtctt ttccgctgaa ccgttccagc aaaaaagact accaacgcaa
      421 tatggattgt cagaatcata taaaagagaa gcaaataact ccttgtcttg tatcaattgc
      481 attataatat cttcttgtta gtgcaatatc atatagaagt catcgaaata gatattaaga
      541 aaaacaaact gtacaatcaa tcaatcaatc atcacataaa atgtctgaat tcatggtttc
      601 caaaggtgaa gagaacaata tggctgtcat caagcccttc atgagattca aggtgcacat
      661 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gcaagcccta
      721 cgagggcacc cagaccgcca agctgcaggt gaccaagggc ggccccctgc ccttcgcctg
      781 ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga
      841 catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggaga gagtgatgaa
      901 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcacctt
      961 catctaccac gtgaagttca tcggcgtgaa cttcccctcc gacggccccg taatgcagaa
     1021 gaagactctg ggctgggagc cctccaccga gagactgtac cccagagacg gcgtgctgaa
     1081 gggcgagatc cacaaggcgc tgaagctgaa gggcggcggc cactacctgg tggagttcaa
     1141 gtca
//
