LOCUS       sequence-124491-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of Sp-dCas9 fused to the VP64 transcription 
            activator, in mammalian cells, under an EF1-alpha promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pEF_dCas9-VP64
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..171)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(202..267)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(274..294)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gatggtgatg atgaccggtt ataacatatc gagatcgaaa tcgtccagag catcggagcc
       61 gagcatgtcc agatcaaagt catccaatgc gtcgcttccc aacatatcca ggtcaaagtc
      121 atcgagggca tcagaaccca gcatgtcgag atcgaaatcg tccagcgcgt cggcgcgccc
      181 gcttccaccg cctcctgcag ccttgtcatc gtcatccttg taatcgatgt catgatcttt
      241 ataatcaccg tcatggtctt tgtagtcgct cgagactttc ctcttcttct tgggggatcc
      301 gtcaccccca agctgtgaca aatctatccg agtttcatat aatcccgtga tggattggtg
      361 aatcagtgtc gcgtctagca cctccttggt agaagtgtat cgtttgcgat ctatcgttgt
      421 gtcaaaatac ttgaatgcgg ctggagcgcc gaggttggta agagtaaaca aatggataat
      481 attttccgcc tgctcacgta tgggtttatc cctgtgcttg ttgtatgcgc ttaatacttt
      541 gtccagattg gcatcagcta ggatgactct cttactgaat tccgaaattt gctctatgat
      601 ttcgtcgaga taatgtttgt gctgctcaac aaaaagttgc ttctgttcgt tatcttcagg
      661 tgaacctttc aacttctcgt aatgggacgc taaatacagg aaattcacgt atttagacgg
      721 tagtgcgagt tcgttcccct tttgaagctc tccggcgcta gccaacatcc gttttcggcc
      781 attttctaac tcaaacagac tatactttgg tagtttaatt atgagatcct tttttacttc
      841 cttgtaacct ttcgcctcaa ggaagtcgat ggggttcttt tcaaaagacg agcgctccat
      901 aatcgttatc cccaataatt ctttgactga cttcagtttc ttggattttc ccttctcaac
      961 ttttgccact actaggacag aatagg
//
