LOCUS       sequence-124492-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of Sp-dCas9 fused to the VP64 transcription 
            activator, in mammalian cells, under an EF1-alpha promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pEF_dCas9-VP64
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..68
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtgtccgcta gagatccgcg gccgcgagag ccgccaccat ggctagcccc aaaaagaaga
       61 ggaaagtgga taaaaagtat tctattggtt tagccatcgg cactaattcc gttggatggg
      121 ctgtcataac cgatgaatac aaagtacctt caaagaaatt taaggtgttg gggaacacag
      181 accgtcattc gattaaaaag aatcttatcg gtgccctcct attcgatagt ggcgaaacgg
      241 cagaggcgac tcgcctgaaa cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa
      301 tatgttactt acaagaaatt tttagcaatg agatggccaa agttgacgat tctttctttc
      361 accgtttgga agagtccttc cttgtcgaag aggacaagaa acatgaacgg caccccatct
      421 ttggaaacat agtagatgag gtggcatatc atgaaaagta cccaacgatt tatcacctca
      481 gaaaaaagct agttgactca actgataaag cggacctgag gttaatctac ttggctcttg
      541 cccatatgat aaagttccgt gggcactttc tcattgaggg tgatctaaat ccggacaact
      601 cggatgtcga caaactgttc atccagttag tacaaaccta taatcagttg tttgaagaga
      661 accctataaa tgcaagtggc gtggatgcga aggctattct tagcgcccgc ctctctaaat
      721 cccgacggct agaaaacctg atcgcacaat tacccggaga gaagaaaaat gggttgttcg
      781 gtaaccttat agcgctctca ctaggcctga caccaaattt taagtcgaac ttcgacttag
      841 ctgaagatgc caaattgcag cttagtaagg acacgtacga tgacgatctc gacaatctac
      901 tggcacaaat tggagatcag tatgcggact tatttttggc tgccaaaaac cttagcgatg
      961 caatcctcct atctgacata ctgagagtta atactgagat taccaaggcg ccgttatccg
     1021 ctt
//
