LOCUS       sequence-124493-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RFP-Atg8 (PAS  marker) with  improved  property.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: PCUP1-DuDre-Atg8-404
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     95..113
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(151..173)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     273..292
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        313..594
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
       61 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
      121 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
      181 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
      241 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
      301 gcaccataga tcaacgacat tactatatat ataatatagg aagcatttaa tagaacagca
      361 tcgtaatata tgtgtacttt gcagttatga cgccagatgg cagtagtgga agatattctt
      421 tattgaaaaa tagcttgtca ccttacgtac aatcttgatc cggagctttt ctttttttgc
      481 cgattaagaa ttaattcggt cgaaaaaaga aaaggagagg gccaagaggg agggcattgg
      541 tgactattga gcacgtgagt atacgtgatt aagcacacaa aggcagcttg gagtatgtct
      601 gttattaatt tcacaggtag ttctggtcca ttggtgaaag tttgcggctt gcagagcaca
      661 gaggccgcag aatgtgctct agattccgat gctgacttgc tgggtattat atgtgtgccc
      721 aatagaaaga gaacaattga cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca
      781 tataaaaata gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa tcaacctaag
      841 gaggatgttt tggctctggt caatgattac ggcattgata tcgtccaact gcatggagat
      901 gagtcgtggc aagaatacca agagttcctc ggtttgccag ttattaaaag actcgtattt
      961 ccaaaagact gcaacatact actcagtgca gcttcacaga aacctcattc gtttattccc
     1021 ttgtttgatt cagaagcagg tgggacaggt gaacttt
//
