LOCUS       sequence-124507-        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration of 2GFP tag at Atg18 C terminus, use auxotrophic marker 
            URA3(Candida albicans).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD218
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(62..778)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(65..778)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga agtggcgcgc
       61 cttatttata taattcatcc ataccatggg taataccagc agcagtaaca aattctaaca
      121 agaccatgtg gtctctcttt tcgtttggat ctttggataa ggcagattga gtggataagt
      181 aatggttgtc tggtaacaag actggaccat caccaattgg agtattttgt tgataatggt
      241 cagctaattg aacagaacca tcttcaatgt tgtgtctaat tttgaagtta actttgatac
      301 cattcttttg tttgtcagcc atgatgtaaa cattgtgaga gttatagttg tattccaatt
      361 tgtgacctaa aatgttacca tcttctttaa aatcaatacc ttttaattcg attctattaa
      421 ctaaggtatc accttcaaac ttgacttcag ctctggtctt gtagttaccg tcatctttga
      481 aaaaaatagt tctttcttga acataacctt ctggcatggc agacttgaaa aagtcatgtt
      541 gtttcatatg atctgggtat ctagaaaaac attgaacacc ataagttaaa gtagtgacta
      601 aggttggcca tggaactggc aatttaccag tagtacaaat aaattttaag gtcaatttac
      661 cgtaagtagc atcaccttca ccttcaccgg agacagaaaa tttgtgacca ttaacatcac
      721 catctaattc aaccaaaatt gggacaacac cagtgaataa ttcttcacct ttagacatga
      781 atggcttctc tagatcacgc gttggaactg gaattttgta caattcatca ataccatggg
      841 taataccagc agcagtaaca aattctaaca agaccatgtg gtctctcttt tcgtttggat
      901 ctttggataa ggcagattga gtggataagt aatggttgtc tggtaacaag actggaccat
      961 caccaattgg agtattttgt tgataatggt cagctaattg aacagaacca tcttcaatgt
     1021 tgtgtctaat tttgaagtta actttgatac cattcttttg tttgtcagcc atgatgtaaa
     1081 cattgtgaga gttatagttg tattccaatt
//
