LOCUS       sequence-124514-        1138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  complementation of yeast auxotrophic marker HIS3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: BS-His3
FEATURES             Location/Qualifiers
     source          1..1138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        134..321
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             322..984
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 cccctcgagg tcgacaagag cttggtgagc gctaggagtc actgccaggt atcgtttgaa
       61 cacggcatta gtcagggaag tcataacaca gtcctttccc gcaattttct ttttctatta
      121 ctcttggcct cctctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt
      181 tttttttcca cctagcggat gactcttttt ttttcttagc gattggcatt atcacataat
      241 gaattataca ttatataaag taatgtgatt tcttcgaaga atatactaaa aaatgagcag
      301 gcaagataaa cgaaggcaaa gatgacagag cagaaagccc tagtaaagcg tattacaaat
      361 gaaaccaaga ttcagattgc gatctcttta aagggtggtc ccctagcgat agagcactcg
      421 atcttcccag aaaaagaggc agaagcagta gcagaacagg ccacacaatc gcaagtgatt
      481 aacgtccaca caggtatagg gtttctggac catatgatac atgctctggc caagcattcc
      541 ggctggtcgc taatcgttga gtgcattggt gacttacaca tagacgacca tcacaccact
      601 gaagactgcg ggattgctct cggtcaagct tttaaagagg ccctaggggc cgtgcgtgga
      661 gtaaaaaggt ttggatcagg atttgcgcct ttggatgagg cactttccag agcggtggta
      721 gatctttcga acaggccgta cgcagttgtc gaacttggtt tgcaaaggga gaaagtagga
      781 gatctctctt gcgagatgat cccgcatttt cttgaaagct ttgcagaggc tagcagaatt
      841 accctccacg ttgattgtct gcgaggcaag aatgatcatc accgtagtga gagtgcgttc
      901 aaggctcttg cggttgccat aagagaagcc acctcgccca atggtaccaa cgatgttccc
      961 tccaccaaag gtgttcttat gtagtgacac cgattattta aagctgcagc atacgatata
     1021 tatacatgtg tatatatgta tacctatgaa tgtcagtaag tatgtatacg aacagtatga
     1081 tactgaagat gacaaggtaa tgcatcattc tatacgtgtc attctgaacg aggcgcgc
//
