LOCUS       sequence-124522-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual-promoter plasmid to express knockdown-proof human TACC3 
            (682-688aa ch-TOG binding site deleted) tagged with EGFP along with 
            shRNA to knock down endogenous TACC3 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
  TITLE     TACC3-ch-TOG track the growing tips of microtubules independently of
            clathrin and Aurora-A phosphorylation.
  JOURNAL   Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.
  PUBMED    25596274
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2015-01-16- 16"; volume: "4"; 
            issue: "2"; pages: "170-9"
COMMENT     Sequence Label: pBrain-GFP-TACC3KDP∆682-688-shTACC3
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
       61 cgggtatgag tctgcaggtc ttaaacgaca aaaatgtcag caatgaaaaa aatacagaaa
      121 attgcgactt cctgttttcg ccaccagaag ttacaggtag gtcatctgtt cttcgtgtgt
      181 cacagaaaga aaatgtgcca cccaagaacc tggccaaagc tatgaaggtg acttttcaga
      241 cacctctgcg ggatccacag acgcacagga ttctaagtcc tagcatggcc agcaaacttg
      301 aggctccttt cactcaggat gacacccttg gactggaaaa ctcacacccg gtctggacac
      361 agaaagagaa ccaacagctc atcaaggaag tggatgccaa aactactcat ggaattctac
      421 agaaaccagt ggaggctgac accgacctcc tgggggatgc aagcccagcc tttgggagtg
      481 gcagctccag cgagtctggc ccaggtgccc tggctgacct ggactgctca agctcttccc
      541 agagcccagg aagttctgag aaccaaatgg tgtctccagg aaaagtgtct ggcagccctg
      601 agcaagccgt ggaggaaaac cttagttcct attccttaga cagaagagtg acacccgcct
      661 ctgagaccct agaagaccct tgcaggacag agtcccagca caaagcggag actccgcacg
      721 gagccgagga agaatgcaaa gcggagactc cgcacggagc cgaggaggaa tgccggcacg
      781 gtgg
//
