LOCUS       sequence-124534-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R1333K mutant SpCas9 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-MT1-NLS-3XHA-NLS
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaagcaggct ggcgccacca tggacaagaa gtactccatt gggctcgata tcggtaccaa
       61 cagcgtcggc tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt
      121 tctgggcaat accgatcgcc acagcataaa gaagaacctc attggagccc tcctgttcga
      181 ctccggggag acggccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg
      241 cagaaagaat cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga
      301 tgactctttc ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga
      361 gcgccaccca atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac
      421 catatatcat ctgaggaaga agctggtaga cagtactgat aaggctgact tgcggttgat
      481 ctatctcgcg ctggcgcaca tgatcaaatt tcggggacac ttcctcatcg agggggacct
      541 gaacccagac aacagcgatg tcgacaaact ctttatccaa ctggttcaga cttacaatca
      601 gcttttcgag gagaacccga tcaacgcatc cggcgttgac gccaaagcaa tcctgagcgc
      661 taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca cagctccctg gggagaagaa
      721 gaacggcctg tttggtaatc ttatcgccct gtcactcggg ctgaccccca actttaaatc
      781 taacttcgac ctgg
//
