LOCUS       sequence-124536-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R1333S mutant SpCas9 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-MT2-NLS-3XHA-NLS
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cggctgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg
       61 caataccgat cgccacagca taaagaagaa cctcattgga gccctcctgt tcgactccgg
      121 ggagacggcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa
      181 gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc
      241 tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca
      301 cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata
      361 tcatctgagg aagaagctgg tagacagtac tgataaggct gacttgcggt tgatctatct
      421 cgcgctggcg cacatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc
      481 agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt
      541 cgaggagaac ccgatcaacg catccggcgt tgacgccaaa gcaatcctga gcgctaggct
      601 gtccaaatcc cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg
      661 cctgtttggt aatcttatc
//
