LOCUS       sequence-124553-       18487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TALENs targeting ANT1 locus, donor molecule with 5' homology 
            arm-Pnos:NptII-35S:ANT13' homology arm as a non-replicating, mutant 
            version of the Gemini Viral Replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18487)
  AUTHORS   Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF
  TITLE     High-frequency, precise modification of the tomato genome.
  JOURNAL   Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
  PUBMED    26541286
REFERENCE   2  (bases 1 to 18487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-6"; volume: "16(1):232. doi"; pages: " 
            10.1186/s13059-015-0796-9"
COMMENT     Sequence Label: pTC151
FEATURES             Location/Qualifiers
     source          1..18487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        956..1301
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1335..1400
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1407..1427
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4761..4817
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4821..4886
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4893..4913
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7644..8231
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      8245..8493
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        9632..9811
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        9664..9847
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             9905..10702
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9962..9981)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10572..10591
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10728..10902
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        11113..11458
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    12210..12234
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             13534..14163
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             14597..15664
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      15730..15924
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(16160..16182)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    16268..16408
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     16323..16342
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(16423..16440)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16594..17182)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16674..16693)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(17269..18063)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtagagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
      121 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
      181 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
      241 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
      301 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
      361 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
      421 caggtatttc cgttggatac gaattattcg tacgaccctc ggcgcgccag atttgccttt
      481 tcaatttcag aaagaatgct aacccacaga tggttagaga ggcttacgca gcaggtatca
      541 tcaagacgat ctacccgagc aataatctcc aggaaatcaa ataccttccc aagaaggtta
      601 aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc
      661 tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcac aaaccaaggc
      721 aagtaataga gattggagtc tctaaaaagg tagttcccac tgaatcaaag gccatggagt
      781 caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac
      841 agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca
      901 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      961 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
     1021 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
     1081 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     1141 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     1201 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     1261 cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggact cctgcaggaa
     1321 aaatggcttc ctccgattat aaggatcacg atggcgacta caaggaccac gatattgact
     1381 acaaagacga tgacgataaa atggctccta agaaaaagcg caaagtcggt atccatggcg
     1441 ttccctctag aatggtggat ctacgcacgc tcggctacag tcagcagcag caagagaaga
     1501 tcaaaccgaa ggtgcgttcg acagtggcgc agcaccacga ggcactggtg ggccatgggt
     1561 ttacacacgc gcacatcgtt gcgctcagcc aacacccggc agcgttaggg accgtcgctg
     1621 tcacgtatca gcacataatc acggcgttgc cagaggcgac acacgaagac atcgttggcg
     1681 tcggcaaaca gtggtccggc gcacgcgccc tggaggcctt gctcacggat gcgggggagt
     1741 tgagaggtcc gccgttacag ttggacacag gccaacttgt gaagattgca aaacgtggcg
     1801 gcgtgaccgc aatggaggca gtgcatgcca gcaggaatgc gttaacaggt gcccccctga
     1861 acctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag caagcgctcg
     1921 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
     1981 tggtggctat cgccagcaac aatggcggca agcaagcgct cgaaacggtg cagcggctgt
     2041 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     2101 acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     2161 accatggcct gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag
     2221 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     2281 accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctcgaa acggtgcagc
     2341 ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc ggaccaagtg gtggctatcg
     2401 ccagccacga tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     2461 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac attggcggca
     2521 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     2581 ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg ctcgaaacgg
     2641 tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg
     2701 ctatcgccag caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     2761 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacggtg
     2821 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     2881 gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg
     2941 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
     3001 tggtggctat cgccagcaac attggcggca agcaagcgct cgaaacggtg cagcggctgt
     3061 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     3121 acattggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     3181 accatggcct gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag
     3241 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     3301 accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctcgaa acggtgcagc
     3361 ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc ggaccaagtg gtggctatcg
     3421 ccagccacga tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     3481 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac attggcggca
     3541 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     3601 ctccggacca agtggtggct atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg
     3661 tgcagcggct gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg
     3721 ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     3781 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacggtg
     3841 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     3901 gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg
     3961 aaagcattgt ggcccagctg agccggcctg atccggcgtt ggccgcgttg accaacgacc
     4021 acctcgtcgc cttggcctgc ctcggcggac gtcctgccat ggatgcagtg aaaaagggat
     4081 tgccgcacgc gccggaattg atcagaagag tcaatcgccg tattggcgaa cgcacgtccc
     4141 atcgcgttgc cggatcccag ctggtgaagt ccgagctgga agaaaaaaag agcgagctgc
     4201 gccacaagct caagtacgtg ccccacgagt acatcgagct gatcgagatc gcccgcaaca
     4261 gcacccaaga ccgcatcctg gagatgaaag tgatggagtt cttcatgaag gtgtacggct
     4321 accgcggcaa gcacctgggc ggctcccgca agcccgatgg cgccatctac accgtgggct
     4381 cccccatcga ctatggcgtc attgtcgaca ccaaggccta ctccggcggc tacaacttac
     4441 ccatcggtca ggccgacgag atgcaacgct acgtgaagga gaaccagacc cgcaataagc
     4501 acattaatcc caacgagtgg tggaaggtgt acccctcctc cgtgaccgag ttcaaattcc
     4561 tgttcgtgtc cggccacttc aagggcaatt ataaggccca actgacccgc ctgaaccgca
     4621 agaccaactg caacggcgcc gtgctgtccg tggaggaact gctgatcggc ggcgagatga
     4681 tcaaggctgg taccctgacc ctggaagagg tgcgccgcaa gttcaacaat ggtgaaatca
     4741 atttcaggtc cggaagcggc gccacgaact tctctctgtt aaagcaagca ggtgacgtgg
     4801 aagaaaaccc agggcccatg gactacaaag accatgacgg tgattataaa gatcatgaca
     4861 tcgattacaa ggatgacgat gacaagatgg cccccaagaa gaagaggaag gtgggcattc
     4921 acggggtgcc ggctagcatg gtggatctac gcacgctcgg ctacagtcag cagcagcaag
     4981 agaagatcaa accgaaggtg cgttcgacag tggcgcagca ccacgaggca ctggtgggcc
     5041 atgggtttac acacgcgcac atcgttgcgc tcagccaaca cccggcagcg ttagggaccg
     5101 tcgctgtcac gtatcagcac ataatcacgg cgttgccaga ggcgacacac gaagacatcg
     5161 ttggcgtcgg caaacagtgg tccggcgcac gcgccctgga ggccttgctc acggatgcgg
     5221 gggagttgag aggtccgccg ttacagttgg acacaggcca acttgtgaag attgcaaaac
     5281 gtggcggcgt gaccgcaatg gaggcagtgc atgcatcgcg caatgcactg acgggtgccc
     5341 ccctgaacct gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag
     5401 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     5461 accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa acggtgcagc
     5521 ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg
     5581 ccagcaacgg tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     5641 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca
     5701 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     5761 ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg ctcgaaacgg
     5821 tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg
     5881 ctatcgccag caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     5941 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacggtg
     6001 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     6061 gcctgacccc ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag caagcgctcg
     6121 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
     6181 tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaacggtg cagcggctgt
     6241 tgccggtgct gtgccaggac catggcctga ctccggacca agtggtggct atcgccagcc
     6301 acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     6361 accatggcct gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag
     6421 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     6481 accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctcgaa acggtgcagc
     6541 ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg
     6601 ccagcaacgg tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     6661 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac attggcggca
     6721 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     6781 ccccggacca agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaacgg
     6841 tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg
     6901 ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     6961 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacggtg
     7021 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     7081 gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag caagcgctcg
     7141 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgact ccggaccaag
     7201 tggtggctat cgccagccac gatggcggca agcaagcgct cgaaacggtg cagcggctgt
     7261 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     7321 acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     7381 accatggcct gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag
     7441 cgctcgaaag cattgtggcc cagctgagcc ggcctgatcc ggcgttggcc gcgttgacca
     7501 acgatcatct ggtggcactc gcgtgcctcg gcggacgtcc tgccatggat gcagtgaaaa
     7561 agggattgcc gcacgcgccg gaattgatca gaagagtcaa tcgccgtatt ggcgaacgca
     7621 cgtcccatcg cgttgccaga tctcaactag tcaaaagtga actggaggag aagaaatctg
     7681 aacttcgtca taaattgaaa tatgtgcctc atgaatatat tgaattaatt gaaattgcca
     7741 gaaattccac tcaggataga attcttgaaa tgaaggtaat ggaatttttt atgaaagttt
     7801 atggatatag aggtaaacat ttgggtggat caaggaaacc ggacggagca atttatactg
     7861 tcggatctcc tattgattac ggtgtgatcg tggatactaa agcttatagc ggaggttata
     7921 atctgccaat tggccaagca gatgaaatgg agcgatatgt cgaagaaaat caaacacgag
     7981 acaaacatct caaccctaat gaatggtgga aagtctatcc atcttctgta acggaattta
     8041 agtttttatt tgtgagtggt cactttaaag gaaactacaa agctcagctt acacgattaa
     8101 atcatatcac taattgtaat ggagctgttc ttagtgtaga agagctttta attggtggag
     8161 aaatgattaa agccggcaca ttaaccttag aggaagtgag gcggaaattt aataacggcg
     8221 agataaactt ttaatagctc gagatatgaa gatgaagatg aaatatttgg tgtgtcaaat
     8281 aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg
     8341 gctttttcta atattaaatg aatgtaagat cacattataa tgaataaaca aatgtttcta
     8401 taatccattg tgaatgtttt gttggatctc ttctgcagca tataactact gtatgtgcta
     8461 tggtatggac tatggaatat gattaaagat aaggagcttt gggtacgtca cgtggctcga
     8521 gcgcgtagtc ctcggtaacc acaacacttg tcggtgagat tatttaatgc tgattagatt
     8581 agacaaaaat taattagttt tgagtagtgg cgtaagtgta aataattagt ctctttttta
     8641 acttagaaaa tagtttaatc cttagtataa atagtcaaaa tcactggaat gaaaaacagt
     8701 ttttaatttt tccaaatttg attctgatac catgttaaat tcgtggttca aaatcactgc
     8761 aatgaaaaga gcaatattgt ttaacttttt ttaggaaaat cgaattgatt tatagtcagt
     8821 tgatatagag tgaatacata aggaacatat acagttgata caattgtata attcgttcat
     8881 acacttaata caaagtgaac ccacaaggaa catatacact taatataatt gtattccttg
     8941 atacaaacca attttgttcg tgtctctact ctctatttca atttcgcttg actctttact
     9001 ttttctaata tgtagctata aatcgtaatt aaacaatact atatctctaa atctcttatt
     9061 aagctcaaac tatggtcata ttcgaaaaaa tccttttaaa tattggtccc ctctcacgat
     9121 taatgatagt tataactaac attcaaattt tagttgtact tgacatctaa aacttaaaaa
     9181 atagtacaag ttaacttttt ctttttttta aaaaaaggaa atacttgtat ttattttttt
     9241 aatatatagt tatatttttg gttatttgaa aatacttgat ctgtcatgta tgctcagtta
     9301 aatatcgtca cattatagag aaaaaagtaa taggagaaaa aaattaaaaa ttatttcgaa
     9361 aaatcaaaat ttttttttga ttgaaatgaa agatgggttt cccaatcgag gctggcagga
     9421 taggtacatt gggaaatttg gatttgtgtg ttgaaaatga ttgttcaatt tggcttttat
     9481 aacatttgtc gtttataatt tgtagaaggc tctctacaag ttggactgaa ggcgggaaac
     9541 gacaatctga tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc
     9601 gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc
     9661 cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc
     9721 aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga
     9781 cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata
     9841 atctgcaccg gatccgctag aggatctcga cctgcaagat cccggggggc aatgagatcc
     9901 taggatgggg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     9961 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
    10021 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
    10081 tgaactccag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
    10141 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
    10201 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
    10261 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
    10321 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
    10381 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
    10441 gcccgacggc gaggatctcg tcgtgacaca tggcgatgcc tgcttgccga atatcatggt
    10501 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
    10561 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    10621 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    10681 ccttcttgac gagttcttct gattaattaa cgatcgacaa gctctatttt ctccataata
    10741 atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg ctcatgtgtt
    10801 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
    10861 ttctaattcc taaaaccaaa atccagtact aaaatccaga tcccccgaat taattcggcg
    10921 ttaattcagc atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac
    10981 tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa
    11041 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
    11101 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
    11161 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
    11221 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
    11281 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
    11341 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
    11401 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agagaacacg
    11461 ggggactctt gacagtagta taatatatta tcaaattatt atgaacagta catctatgtc
    11521 ttcattggga gtgagaaaag gttcatggac tgatgaagaa gattttcttc taagaaaatg
    11581 tattgataag tatggtgaag gaaaatggca tcttgttccc ataagagctg gtaactatta
    11641 aattaactat cacgttattt ttatttgtct ttctgtctca ttttatttga cgttattacg
    11701 aatatcatct gaaaatgtac gtgcaggtct gaatagatgt cggaaaagtt gtagattgag
    11761 gtggctgaat tatctaaggc cacatatcaa gagaggtgac tttgaacaag atgaagtgga
    11821 tctcattttg aggcttcata agctcttagg caacaggcat gcaagtttat gttttgacaa
    11881 aatttgatta gtatatatta tatatacgtg tgactatttc atctaaatgt tacgttattt
    11941 tacgtagatg gtcacttatt gctggtagac ttcccggaag gacagctaac gatgtgaaaa
    12001 actattggaa cactaatctt ctaaggaagt taaatactac taaaattgtt cctcgcgaaa
    12061 agattaacaa taagtgtgga gaaattagta ctaagattga aattataaaa cctcaacgac
    12121 gcaagtattt ctcaagcaca atgaagaatg ttacaaacaa taatgtaatt ttggacgagg
    12181 aggaacattg catttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
    12241 gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc
    12301 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
    12361 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
    12421 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
    12481 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
    12541 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
    12601 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
    12661 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
    12721 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
    12781 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
    12841 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
    12901 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
    12961 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
    13021 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
    13081 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
    13141 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
    13201 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
    13261 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
    13321 gccgcccgcg cacggctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
    13381 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
    13441 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
    13501 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
    13561 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
    13621 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
    13681 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
    13741 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
    13801 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
    13861 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
    13921 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
    13981 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
    14041 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
    14101 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
    14161 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
    14221 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
    14281 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
    14341 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
    14401 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
    14461 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
    14521 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc
    14581 gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
    14641 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
    14701 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
    14761 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
    14821 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
    14881 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
    14941 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
    15001 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
    15061 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
    15121 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
    15181 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
    15241 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
    15301 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
    15361 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
    15421 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
    15481 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
    15541 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
    15601 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
    15661 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
    15721 cctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
    15781 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
    15841 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
    15901 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
    15961 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
    16021 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
    16081 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
    16141 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggaaacggtc acagcttgtc
    16201 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    16261 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
    16321 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    16381 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
    16441 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    16501 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    16561 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    16621 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    16681 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    16741 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    16801 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    16861 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    16921 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    16981 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    17041 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    17101 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    17161 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    17221 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc
    17281 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
    17341 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
    17401 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
    17461 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
    17521 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
    17581 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
    17641 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
    17701 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
    17761 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
    17821 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
    17881 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
    17941 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
    18001 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
    18061 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
    18121 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
    18181 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
    18241 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
    18301 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
    18361 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
    18421 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
    18481 tggctgg
//
