LOCUS       sequence-124556-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-J3
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      606..637
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 actctctact gtttctccat acccgttttt ttggacgcgt acaactcaag tctgacataa
       61 atgaccgcta tggcaagata tttgggtcct aagctcaagc tgagccgtcg tgagggcacc
      121 gacttattcc ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat tgaacaagct
      181 cctggccagc acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca gttgcgtgaa
      241 aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa ctactacaaa
      301 gaagcagcac gtctgaaagg caacaccggt gaaaacctgt tggctctgct ggaaggtcgt
      361 ctggacaacg ttgtataccg tatgggcttc ggtgccactc gtgcagaagc acgtcagctg
      421 gttagccata aagcaattat ggtaaacggt cgtgttgtta acatcgcttc ttatcaggtt
      481 agtccgaatg acgttgtaag cattcgtgag aaagcgaaga agcagtctcg cgtgaaagcc
      541 gctctggagc tggctgagca gcgtgaaaag ccaacctaaa cttaattaac ggcactcctc
      601 agccaagtca aaagcctccg accggaggct tttgactaca tgcccatggc gtttacgccc
      661 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
      721 catcacaaac ggcatgatga acctga
//
