LOCUS       sequence-124564-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..49
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccatcgattc gaattcaagg atcaacaagt ttgtacaaaa aagcaggctg gcgccaccat
       61 ggacaagaag tactccattg ggctcgatat cggtaccaac agcgtcggct gggccgtcat
      121 tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca
      181 cagcataaag aagaacctca ttggagccct cctgttcgac tccggggaga cggccgaagc
      241 cacgcggctc aaaagaacag cacggcgcag atatacccgc agaaagaatc ggatctgcta
      301 cctgcaggag atctttagta atgagatggc taaggtggat gactctttct tccataggct
      361 ggaggagtcc tttttggtgg aggaggataa aaagcacgag cgccacccaa tctttggcaa
      421 tatcgtggac gaggtggcgt accatgaaaa gtacccaacc atatatcatc tgaggaagaa
      481 gctggtagac agtactgata aggctgactt gcggttgatc tatctcgcgc tggcgcacat
      541 gatcaaattt cggggacact tcctcatcga gggggacctg aacccagaca acagcgatgt
      601 cgacaaactc tttatccaac tggttcagac ttacaatcag cttttcgagg agaacccgat
      661 caacgcatcc ggcgttgacg ccaaagcaat cctgagcgct aggctgtcca aatcccggcg
      721 gctcgaaaac ctcatcgcac agctccctgg ggagaagaag aacggcctgt ttggtaatct
      781 tatcgccctg tcactcgggc tgacccc
//
